THP-1 cells were seeded in 6 cm dishes at a density of 1 × 10*6 cells/dish for transfection. Transfection was performed with 1.6 µg/ml (final concentration) of Lipofectamine 2000 (Invitrogen) and 20 µM (final concentration) of stealth siRNA (Invitrogen) or negative control (Stealth<sup>TM</sup> RNAi Negative Control Medium GC Duplex --- catalog number 12935-300, Invitrogen) by reverse transfection protocol in accordance with the manufacturer’s instructions.
<HTML><HEAD><TITLE>KnockDown by siRNA</TITLE><meta http-equiv="content-type" content="text/html; charset=UTF-8"/><script type="text/javascript" src="/sw/js/panel/panelDescription.js"></script></HEAD><BODY>"THP-1 cells were seeded in 6 cm dishes at a density of 1 × 10*6 cells/dish for transfection. Transfection was performed with 1.6 µg/ml (final concentration) of Lipofectamine 2000 (Invitrogen) and 20 µM (final concentration) of stealth siRNA (Invitrogen) or negative control (Stealth<sup>TM</sup> RNAi Negative Control Medium GC Duplex --- catalog number 12935-300, Invitrogen) by reverse transfection protocol in accordance with the manufacturer’s instructions."</BODY></HTML>
KnockDown by siRNA