PAFA_BIFLS BLIJ_2201 Pup-conjugating enzyme Blon_2124 485 Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine (By similarity). Proteasome accessory factor A MPQLRDSGTRSLHATEPVPSAEMDGFCRIFGVETEYGVAVTGAERPVDAGQVAMTMFQPIVSRSRSTNTYLANGSRLYLDVGSHPEYATAEARDPREALGQDLAGEHVMRNLALKAQRKLRESYSEHATIHVFKNNVDSAGHAFGCHENYLVRRFVPLETIEHQLLPFLITRQLYTGAGRMTPDGFQITQRADFLDEAVSSATTRSRPMVNTRDEPHADPDSFRRLHVIIGDSNRSQWSTWMKLAVTHLVLCAIEDAFRLGTPSGFEHCAFADPAAANRTVSRFLDDPHAELTLESGESVSALGLQRRYYAAVKAFIETHGDALASSLPATTIVTIMGEWSRVLDALERGAYDALADRVDWAAKKRLFDALKRRRPDVTFAQMEQLELDYHDIANGRLYGSLTSRNQMRELLTGDDVEYAVHNPPTDTRAALRGRFVDAALNVGAQFSADWTHLTLTAPERREAILLDPFEAEPTPEFEQLMEAL pafA Pup--protein ligase