pafA BL1797 Proteasome accessory factor A Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine (By similarity). PAFA_BIFLO 486 MPQLRDSGTRSLHATEPVPSAEMDGFCRIFGVETEYGVAVTGAERPVDAGQVAMTMFQPIVSRSRSTNTYLANGSRLYLDVGSHPEYATAEARDPREALAQDLAGEHVMRNLALKAQRKLRESYGAHATIHVFKNNVDSAGHAFGCHENYLVRRFVPLETIEHQLLPFLITRQLYTGAGRMTPDGFQITQRADFLDEAVSSATTRSRPMVNTRDEPHADPDSFRRLHVIIGDSNRSQWSTWMKLAVTHLVLCAIEDAFRHGVPSGFEQYAFADPAAANRTVSRFLDNPRAELTLESGESVSALGLQRRYYAAVKAFIETHGDALAGSLPATTIDTIMGEWSRVLDALERGAYDALADRVDWAAKKCLFDALKRRRPDVTFAQMEQLELDYHDIANGRLYGSLVARNQMRELLTGDNVEYAVHNPPTDTRAALRGRFVDAALNVGAQFSADWTHLTLTAPERREAILLDPFEAEPTLGFEQLMEALN Pup--protein ligase Pup-conjugating enzyme