Lapik Yevgeniya R 7 Heterotrimeric G proteins are implicated in diverse signaling processes in plants, but the molecular mechanisms of their function are largely unknown. Finding G protein effectors and regulatory proteins can help in understanding the roles of these signal transduction proteins in plants. A yeast two-hybrid screen was performed to search for proteins that interact with Arabidopsis G protein alpha-subunit (GPA1). One of the identified GPA1-interacting proteins is the cupin-domain protein AtPirin1. Pirin is a recently defined protein found because of its ability to interact with a CCAAT box binding transcription factor. The GPA1-AtPirin1 interaction was confirmed in an in vitro binding assay. We characterized two atpirin1 T-DNA insertional mutants and established that they display a set of phenotypes similar to those of gpa1 mutants, including reduced germination levels in the absence of stratification and an abscisic acid-imposed delay in germination and early seedling development. These data indicate that AtPirin1 likely functions immediately downstream of GPA1 in regulating seed germination and early seedling development. Kaufman Lon S 1578-90 15 12837948 2003 Jul The Arabidopsis cupin domain protein AtPirin1 interacts with the G protein alpha-subunit GPA1 and regulates seed germination and early seedling development. Lapik Yevgeniya R et al. 2003 Jul. Plant Cell 15(7):1578-90.