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    <ns5:pria35u13i xml:lang="en">Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for pollen tube growth.</ns5:pria35u13i>
    <rdfs:label xml:lang="en">Hashida Shin-nosuke et al. 2007 Feb. Plant J. 49(4):694-703.</rdfs:label>
    <ns5:pria35u8i xml:lang="en">17270012</ns5:pria35u8i>
    <ns5:pria35u3i xml:lang="en">Uchimiya Hirofumi</ns5:pria35u3i>
    <ns5:pria35u6i xml:lang="en">4</ns5:pria35u6i>
    <ns4:attributionURL rdf:resource="http://www.ncbi.nlm.nih.gov/pubmed/17270012"/>
    <ns5:pria35u3i xml:lang="en">Takahashi Hideyuki</ns5:pria35u3i>
    <ns5:pria35u7i xml:lang="en">694-703</ns5:pria35u7i>
    <ns5:pria35u3i xml:lang="en">Kawai-Yamada Maki</ns5:pria35u3i>
    <ns5:pria35s10i xml:lang="en">While mammals and fungi possess nicotinate/nicotinamide mononucleotide adenyltransferase (NMNAT) isoforms, Arabidopsis thaliana only contains a single NMNAT gene, AtNMNAT (At5g55810). We analyzed the enzymatic activity of the AtNMNAT-encoded protein to determine the role of AtNMNAT in plant development. AtNMNAT catalyzed the synthesis of nicotinate adenine dinucleotide (NaAD) from nicotinate mononucleotide (NaMN) in the Preiss-Handler-dependent pathway, and of nicotinamide adenine dinucleotide (NAD) from nicotiamide mononucleotide (NMN) in the Preiss-Handler-independent pathway. Prominent AtNMNAT expression was detected in the male gametophyte. Moreover, AtNMNAT expression was spatio-temporally regulated during microspore development and pollen tube growth. Disruption of the AtNMNAT gene (atnmnat mutant) was characterized by a decrease in NAD content in pollen. Cytological examinations revealed that the atnmnat mutant was gametophytically impaired in in vivo and in vitro pollen tube growth. Our results suggest that metabolic fulfillment via the NAD pathway is indispensable for normal pollen growth and subsequent normal seed production.</ns5:pria35s10i>
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    <ns5:pria35u9i xml:lang="en">2007 Feb</ns5:pria35u9i>
    <ns5:pria35u3i xml:lang="en">Hashida Shin-nosuke</ns5:pria35u3i>
    <ns5:pria35u5i xml:lang="en">49</ns5:pria35u5i>
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