18308-13 Higher plant species differ widely in their growth responses to ammonium (NH(4)(+)). However, the molecular genetic mechanisms underlying NH(4)(+) sensitivity in plants remain unknown. Here, we report that mutations in the Arabidopsis gene encoding GDP-mannose pyrophosphorylase (GMPase) essential for synthesizing GDP-mannose confer hypersensitivity to NH(4)(+). The in planta activities of WT and mutant GMPases all were inhibited by NH(4)(+), but the magnitude of the inhibition was significantly larger in the mutant. Despite the involvement of GDP-mannose in both l-ascorbic acid (AsA) and N-glycoprotein biosynthesis, defective protein glycosylation in the roots, rather than decreased AsA content, was linked to the hypersensitivity of GMPase mutants to NH(4)(+). We conclude that NH(4)(+) inhibits GMPase activity and that the level of GMPase activity regulates Arabidopsis sensitivity to NH(4)(+). Further analysis showed that defective N-glycosylation of proteins, unfolded protein response, and cell death in the roots are likely important downstream molecular events involved in the growth inhibition of Arabidopsis by NH(4)(+). Qian Weiqiang Qin Cheng et al. 2008 Nov. Proc. Natl. Acad. Sci. U.S.A. 105(47):18308-13. GDP-mannose pyrophosphorylase is a genetic determinant of ammonium sensitivity in Arabidopsis thaliana. 105 Wang Daowen Wang Wenfeng Wu Yue Yu Chunmei Qin Cheng Jiang Xinhang 47 19011088 Wu Ping 2008 Nov