<p>Pyruvate formate lyase-activating enzyme <db_xref db="EC" dbkey="1.97.1.4"/> is a 28 kDa monomeric protein which is involved in the anerobic glucose metabolism pathway. It is a member of the radical S-adenosylmethionine family of enzymes which initiate radical catalysis. It is necessary to activate pyruvate formate lyase (PFL), which catalyses the conversion of pyruvate and coenzyme A to formate and acetyl CoA.</p><p>Pyruvate formate lyase activating enzyme functions by catalysing the formation of the Gly734 radical of PFL. It utilises a [4Fe-4S] cluster and adenosylmethionine to generate a putative adenosyl radical, which extracts a hydrogen atom from Gly734 on PFL, thus activating PFL for catalysis [<cite idref="PUB00044952"/>]. Members of this family are iron-sulphur proteins with a radical-SAM domain.</p> Pyruvate formate-lyase activating enzyme