ERK1/2 MAP kinase <p>Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. Phosphoprotein phosphatases catalyse the reverse process. Protein kinases fall into three broad classes, characterised with respect to substrate specificity [<cite idref="PUB00005115"/>]:</p><p> <ul> <li>Serine/threonine-protein kinases</li><li>Tyrosine-protein kinases</li><li>Dual specific protein kinases (e.g. MEK - phosphorylates both Thr and Tyr on target proteins)</li> </ul> </p><p>Protein kinase function has been evolutionarily conserved from <taxon tax_id="562">Escherichia coli</taxon> to human [<cite idref="PUB00020114"/>]. Protein kinases play a role in a multitude of cellular processes, including division, proliferation, apoptosis, and differentiation [<cite idref="PUB00015362"/>]. Phosphorylation usually results in a functional change of the target protein by changing enzyme activity, cellular location, or association with other proteins. The catalytic subunits of protein kinases are highly conserved, and several structures have been solved [<cite idref="PUB00034898"/>], leading to large screens to develop kinase-specific inhibitors for the treatments of a number of diseases [<cite idref="PUB00034899"/>].</p><p>MAP (Mitogen Activated Protein) kinases participate in kinase cascades,whereby at least 3 protein kinases act in series, culminating in activationof MAP kinase [<cite idref="PUB00007546"/>, <cite idref="PUB00011603"/>]. MAP kinases are activated by dual phosphorylationon both tyrosine and threonine residues of a conserved TXY motif.</p><p>ERKs (Extracellularly Regulated Kinases) belong to the family of MAPkinases. ERK1 and ERK2, proteins of 43 and 41kDa respectively, are ~85%identical, even higher levels of similarity being seen in substrate bindingregions. Both are ubiquitously expressed, although levels vary fromtissue to tissue. They are activated, for example, by serum, growth factorsand cytokines. They phosphorylate both cytoskeletal proteins andtranscription factors, resulting ultimately in cell growth. Substrates carrya PX(T/S)P motif; other such "docking domains" also exist within ERK, whichinteract with specific motifs in the substrate: e.g., ERK1 and ERK2 possess2 DXXD docking sites capable of interaction with a Kinase Interaction Motif(KIM), which can be found on activators (MAPKK), inhibitors (PTP-SL and dualspecificity phosphatases) and substrates (Elk-1).</p>