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  <rdf:Description rdf:about="http://metadb.riken.jp/db/SciNetS_rib220i/crib220u228rib220u111i">
    <ns0:prib220u291i>
      <ns0:crib220u270i rdf:about="http://metadb.riken.jp/db/SciNetS_rib220i/crib220u270rib220u1883i">
        <ns0:prib220u288i xml:lang="en">5</ns0:prib220u288i>
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        <rdfs:label xml:lang="en">crib220u270rib220u1883i</rdfs:label>
        <ns0:prib220u307i xml:lang="en">Column chromatography</ns0:prib220u307i>
        <ns0:prib220u311i xml:lang="en">buffer (180 ml) to buffer + 0.3 M NaCl (180 ml)</ns0:prib220u311i>
        <ns0:prib220u286i xml:lang="en">20 mM Tris-HCl, pH 8.0</ns0:prib220u286i>
        <ns0:prib220u268i xml:lang="en">18</ns0:prib220u268i>
        <foaf:depiction rdf:resource="http://metadb.riken.jp/dbfiles/SciNetS_rib220i/crib220u270i/crib220u270rib220u1883i/M00021-01-02-02-7.jpg"/>
        <ns0:prib220u205i xml:lang="en">0.11 M NaCl</ns0:prib220u205i>
        <ns0:prib220u257i xml:lang="en">2</ns0:prib220u257i>
        <ns0:prib220u240i xml:lang="en">7</ns0:prib220u240i>
        <ns0:prib220u330i xml:lang="en">_</ns0:prib220u330i>
        <ns0:prib220u298i xml:lang="en">anion exchange column chromatography (GE Healthcare UK Ltd., RESOURCE Q)</ns0:prib220u298i>
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