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      <ns1:crib220u270i rdf:about="http://metadb.riken.jp/db/SciNetS_rib220i/crib220u270rib220u62608i">
        <ns1:prib220u205i xml:lang="en">0.14 M NaCl</ns1:prib220u205i>
        <ns1:prib220u268i xml:lang="en">6</ns1:prib220u268i>
        <ns1:prib220u330i xml:lang="en">_</ns1:prib220u330i>
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        <ns1:prib220u311i xml:lang="en">buffer (45 ml) to buffer + 0.4 M NaCl (45 ml)</ns1:prib220u311i>
        <ns1:prib220u286i xml:lang="en">20 mM Tris-HCl, pH 8.0</ns1:prib220u286i>
        <ns1:prib220u240i xml:lang="en">8</ns1:prib220u240i>
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        <ns1:prib220u257i xml:lang="en">4</ns1:prib220u257i>
        <ns1:prib220u288i xml:lang="en">2</ns1:prib220u288i>
        <ns1:prib220u298i xml:lang="en">anion exchange column chromatography (GE Healthcare UK Ltd., RESOURCE Q)</ns1:prib220u298i>
        <ns1:prib220u307i xml:lang="en">Column chromatography</ns1:prib220u307i>
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