The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14).
E. coli neo, mouse p38 genomic DNA
条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>公表を前提とした学術研究に限る。共著を必要とする共同研究は寄託後2年間に限定する。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。
理化学研究所
B6.129-Mapk14<tm1Mka>(118)/Rbrc
R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Activity of the p38alpha MAP kinase is stimulated by various stresses and hematopoietic growth factors. This strain was created to investigate the role of p38alpha in mouse development and physiology (Tamura et al. 200). Some p38alpha (-/-) embryos die between embryonic days 11.5 and 12.5, those that develop past this stage have normal morphology but are anemic owing to failed definitive erythropoiesis, caused by diminished erythropoietin (Epo) gene expression.
This B6 congenic strain was created by Dr. Sudo T to obtainp38alpha-deficient mice with standard and stable genetic background. The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14) at the animal facility of RIKEN Tsukuba Institute.
RBRC00361
Tatsuhiko SUDO
RIKEN
Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol>
須藤 龍彦
C (3-6 months)
true
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.<br>The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. <br>The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
<a href='https://brc.riken.jp/mus/pcr00361'>Genotyping protocol -PCR-</a>
C(3〜6か月)
C57BL/6-TgH(p38)/118
C57BL/6-TgH(p38)/118
C57BL/6-TgH(p38)/118
This B6 congenic strain was created by Dr. Sudo T to obtain p38alpha-deficient mice with standard and stable genetic background. The mice have been successively backcrossed to C57BL/6JJcl strain for 14 generations (N14) at the animal facility of RIKEN Tsukuba Institute.