条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>共同研究 Heterozygote x Wild-type [C57BL/6JJcl] B6.129P2-Cdkn1b<tm1Kin>; p27 Knockout mice B6.129P2-Cdkn1b<tm1Kin>; p27 Knockout mice Heterozygote x Wild-type [C57BL/6JJcl] 九州大学 p27 (Cdkn1b) gene knockout mice. A 2.5 kb region containing exons 1 and 2 was replaced with a PGK-neo cassette. C（3〜6か月） p27 (Cdkn1b) 遺伝子のノックアウトマウス。エクソン1と2を含む2.5 kb領域をPGK-neoカセットで置換。, <a href='https://brc.riken.jp/mus/pcr00458'>Genotyping protocol -PCR-</a> RBRC00458 Washington University School of Medicine・中山敬一先生（1996）。E14 ES細胞を用いて作出。C57BL/6へ戻し交配された。 Keiichi NAKAYAMA C (3-6 months) E14 [129P2/OlaHsd] mouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptII) gene of the transposon Tn5 in Escherichia coli K12), mouse PGK polyA, mouse Cdkn1b genomic DNA p27 (Cdkn1b) 遺伝子のノックアウトマウス。エクソン1と2を含む2.5 kb領域をPGK-neoカセットで置換。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.bioreg.kyushu-u.ac.jp/saibou/index_en.html" target="_blank">Lab HP</A> 中山　敬一 Kyushu Univ. Developed by Keiko Nakayama and Kei-ichi Nakayama, Howard Hughes Medical Institute, Washington University School of Medicine in 1996. E14 ES cells were used to generate the mutant mice. The mutant mice were backcrossed to C57BL/6. B6.129P2-Cdkn1b<tm1Kin> B6.129P2-Cdkn1b<tm1Kin> true