C.Cg-Stam<tm1Sug> Stam2<tm1Sug>, STAM1+/-, STAM2+/- C.Cg-Stam<tm1Sug> Stam2<tm1Sug>, STAM1+/-, STAM2+/- Neurobiology Research Kazuo SUGAMURA 条件を付加する。<br>利用者は事前に寄託者に利用の報告をする。公表を前提とした学術研究に限る。利用者が当該リソースを使用した研究成果に基づく特許等の申請を行う場 合は、寄託者と別途協議を行う。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse STAM1 and STAM2 genomic DNA Developed by Kazuo Sugamura, Tohoku University Graduate School of Medicine in 1999. The mutant mice were backcrossed to BALB/c over 6 times. C (3-6 months) C.Cg-Stam<tm1Sug> Stam2<tm1Sug>/SugRbrc C.Cg-Stam<tm1Sug> Stam2<tm1Sug>/SugRbrc The RECIPIENT of BIOLOGICAL RESOURCE shall prior inform the DEPOSITOR of the purpose of the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. STAM1, STAM2 double knockout mice. STAM1 homozygous mutant mice show growth defect with aging, and neurological abnormality (reduction of pyramidal cells in CA3 of the hippocampus). STAM2 homozygous mutant mice are indistinguishable from controls. Double homozygous mutant mice are embryonic lethal. C（3〜6か月） STAM1/STAM2ダブルノックアウトマウス。STAM1ホモノックアウトマウスは加齢とともに成長不良。末梢神経反射異常を呈す。組織学的には海馬CA3領域特異的神経細胞の脱落をみる。STAM2ホモノックアウトマウスは特に異常は認められない。STAM1/STAM2ダブルホモマウスは胎生致死。 Tohoku Univ. 東北大学 Cre/loxP system RBRC00672 J1 [129S4/SvJae] 菅村　和夫 <a href='https://brc.riken.jp/mus/pcr00672'>Genotyping protocol -PCR-</a> 東北大学大学院医学系研究科・菅村和夫先生（1999）。BALB/c背景（N6以上）。 Stam: Heterozygote x Wild-type [or Crossing to BALB/cCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to BALB/cCrSlc] Stam: Heterozygote x Wild-type [or Crossing to BALB/cCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to BALB/cCrSlc]