Tadahiro SHIOMI Sibling Mating (Heterozygote x Wild-type)Homozygous mutant mice display severely postnatal growth failure and underwent premature death. Natl. Inst. Radiol. Sci. <a href='https://brc.riken.jp/mus/pcr00807'>Genotyping protocol -PCR-</a> C（3〜6か月） B6.129S2-Ercc5<tm1Shm>. Xeroderma pigmentosum group G (XPG) gene is a structure-specific DNA endonuclease which plays a role in nucleotide exision repair and transcription-coupled repair of oxidative DNA damage. Xeroderma pigmenrosum (XP) is a autosomal recessive disorder characterized by hypersensitivity to sunlight, abnomal pigmentation, and predisposition to skin cancers, and caused by defects in an early step of the nucleotide exicision repair pathway. Xpg2 deficient mutant mice exhibit severely postnatal growth failure and underwent premature death. This strain is useful as a animal model for Xeroderma Pigmentosum. Cell Biology Research Developed by Dr. T. Shiomi, National Institute of Radiological sciences in 1999. A neomycin selected cassette was transfered into D3 ES cels to replace the exon 3 of Ercc5 gene. C57BL/6Cr background. D3 [129S2/SvPas] 放射線医学総合研究所 herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Xpg genomic DNA 色素乾皮症G群モデルマウス。ホモ個体は生長阻害が認められ、離乳期頃迄に死亡。由来細胞はヌクレオチド修復欠損。 C (3-6 months) Xpg/2 Knockout mice ホモ個体は離乳期迄に死亡する為、継代不可。ヘテロで維持する。ヘテロ個体とC57BL/6を交配する。ヘテロ個体はC57BL/6と同様の繁殖効率を示す。 条件を付加する。<br>研究成果の公表にあたって謝辞の表明を必要とする。 塩見　忠博 true B6.129S2-Ercc5<tm1Shm>/ShmRbrc RBRC00807 Mouse Models for Human Disease Xpg/2 Knockout mice Xpg/2 Knockout mice ES細胞のターゲテイング　放医研、阪大微生物研にて キメラマウス作製、放医研に導入後、同研究所にて飼育を続ける。 Sibling Mating (Heterozygote x Wild-type) Homozygous mutant mice display severely postnatal growth failure and underwent premature death. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.