<a href='https://brc.riken.jp/mus/pcr00821'>Genotyping protocol -PCR-</a> Kuniya ABE mouse Oct 3/4 genomic DNA, Jellyfish GFP cDNA, SV40 polyA Developed by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 1999. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. Line 18 (RBRC00821) and Line 20 (RBRC00822). Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) by deletion in the proximal enhancer (PE). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development. 阿部　訓也 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). <br>寄託後１年間は、共著を必要とする共同研究とする。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。 Oct-delta PE-GFP #18, TgN(deGFP)18Imeg Oct-delta PE-GFP #18, TgN(deGFP)18Imeg true 理化学研究所筑波研究所 C (3-6 months) RBRC00821 <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/sep_2005_mm" target="_blank">Mouse of the Month Sep 2005</A> Developmental Biology Research RIKEN,Tsukuba C（3〜6か月） POU転写因子Oct-4 (Pou5f1) 遺伝子を含む18kbのゲノム断片 (Genomic Oct-4 Fragment of 18) とGFP遺伝子との融合遺伝子を導入したトランスジェニックマウス。GOF18のproximal enhancer (PE) 部は除去されている。始原生殖細胞・未分化全能性細胞特異的にGFPを発現する。 Carrier x Noncarrier [C57BL/6JJcl] Carrier x Noncarrier [C57BL/6JJcl] The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC. <br>Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. Reproductive Biology Research Fluorescent Proteins/lacZ System Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://mcd.brc.riken.jp/en/" target="_blank">Lab HP</A> 理化学研究所バイオリソースセンター（熊本大学発生医学研究センター）・阿部訓也先生（1999）。C57BL/6J受精卵にTgのインジェクションにより作出。ライン18（RBRC00821）、ライン20（RBRC00822）。 C57BL/6J-Tg(deGFP)18Imeg C57BL/6J-Tg(deGFP)18Imeg