<rdf:RDF
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:ns5="http://nbrp.jp/ontology/"
    xmlns:ns1="http://metadb.riken.jp/db/rikenbrc_mouse/"
    xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#"
    xmlns:ns11="http://purl.org/net/cito/"
    xmlns:ns6="http://metadb.riken.jp/db/bioresource_schema/"
    xmlns:ns4="http://metadb.riken.jp/terms/xsearch#"
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:ns9="http://purl.obolibrary.org/obo/"
    xmlns:ns14="http://mus.brc.riken.jp/DataSheet/"
    xmlns:skos="http://www.w3.org/2004/02/skos/core#"
    xmlns:ns7="http://semanticscience.org/resource/"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#">
  <rdfs:Class rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC00869">
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns4:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Growth. Differ., 41, 675-684 (1999).</ns4:termsAndConditions>
    <dcterms:identifier>RBRC00869</dcterms:identifier>
    <rdfs:subClassOf rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/Strain"/>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
    <ns1:genotypingProtocol rdf:resource="http://mus.brc.riken.jp/DataSheet/pcr00869"/>
    <ns6:brs_depositor xml:lang="ja">松居　靖久</ns6:brs_depositor>
    <ns6:brs_hasAllele rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/brc_allele79"/>
    <ns1:strainDevelopment xml:lang="ja">University of Pennsylvania・Hans R. Schoeler、大阪府立母子健康総合医療センター研究所・松居靖久先生（1997）。B6D2F1受精卵にTgのインジェクションにより作出。C57BL/6へ戻し交配された系統（N3以上）（RBRC00868、RBRC01198）。129背景へ戻し交配された系統（N7）（RBRC01198）（理化学研究所バイオリソースセンター・小倉淳郎先生）。</ns1:strainDevelopment>
    <ns1:colonyMaintenance xml:lang="en">Carrier x Noncarrier [C57BL/6JJcl]</ns1:colonyMaintenance>
    <ns1:colonyMaintenance xml:lang="ja">Carrier x Noncarrier [C57BL/6JJcl]</ns1:colonyMaintenance>
    <rdfs:label>B6.Cg-Tg(GOF18dPE/EGFP)48Ymat</rdfs:label>
    <rdfs:label xml:lang="en">B6.Cg-Tg(GOF18dPE/EGFP)48Ymat</rdfs:label>
    <dc:description xml:lang="ja">POU転写因子Oct-4 (Pou5f1) 遺伝子を含む18kbのゲノム断片 (Genomic Oct-4 Fragment of 18) とGFP遺伝子との融合遺伝子を導入したトランスジェニックマウス (Dev Growth Differ 1999) 。Oct-4遺伝子は始原生殖細胞特異的に発現する遺伝子であり、始原生殖細胞 (PCG) を蛍光により区別、観察することが可能。GOF18のproximal enhancer (PE) 部を含むライン (RBRC00868) 、PEを除去したライン (RBRC00869とRBRC01198) 。,</dc:description>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/10646797"/>
    <ns6:brs_depositorOrganization xml:lang="en">Osaka Med. Ctr. Maternal Child Health, Immunol.</ns6:brs_depositorOrganization>
    <ns1:strainDevelopment xml:lang="en">Developed by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997.  The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.  The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198).  Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center).</ns1:strainDevelopment>
    <ns6:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_3"/>
    <ns9:RO_0002162 rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10090"/>
    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
    <ns6:brs_hasAppearance rdf:parseType="Resource">
    </ns6:brs_hasAppearance>
    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/animal/detail.php?brc_no=RBRC00869"/>
    <ns4:application>Developmental Biology Research</ns4:application>
    <ns5:organism rdf:parseType="Resource">
    </ns5:organism>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_8"/>
    <ns6:brs_depositor xml:lang="en">Yasuhisa MATSUI</ns6:brs_depositor>
    <ns4:termsAndConditions xml:lang="en">In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth. Differ., 41, 675-684 (1999).</ns4:termsAndConditions>
    <ns1:isRecombinant rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean"
    >true</ns1:isRecombinant>
    <dc:description xml:lang="ja">POU転写因子Oct-4 (Pou5f1) 遺伝子を含む18kbのゲノム断片 (Genomic Oct-4 Fragment of 18) とGFP遺伝子との融合遺伝子を導入したトランスジェニックマウス (Dev Growth Differ 1999) 。Oct-4遺伝子は始原生殖細胞特異的に発現する遺伝子であり、始原生殖細胞 (PCG) を蛍光により区別、観察することが可能。GOF18のproximal enhancer (PE) 部を含むライン (RBRC00868) 、PEを除去したライン (RBRC00869とRBRC01198) 。</dc:description>
    <ns6:brs_geneticBackground rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/BackgroundStrain_32"/>
    <skos:altLabel xml:lang="ja">B6.D2-Tg(GOF18δPE/EGFP)48, GOF18-δPE-GFP line48 transgenic mouse</skos:altLabel>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/straintype_2"/>
    <ns4:application>Fluorescent Proteins/lacZ System</ns4:application>
    <ns4:resourceTypeLink rdf:resource="http://metadb.riken.jp/terms/xsearch#resource_type_transgene"/>
    <ns6:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_2"/>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/10646797"/>
    <ns1:donorDNA>Oct 3 promoter, Jellyfish GFP cDNA</ns1:donorDNA>
    <ns6:brs_depositedBy rdf:parseType="Resource">
    </ns6:brs_depositedBy>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns4:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;GFP Transfer License (&lt;A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude"&gt;English&lt;/A&gt;)&lt;br&gt;Please fill in the &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc"&gt;Schedule A&lt;/A&gt;, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"&gt; Schedule B&lt;/A&gt;. &lt;/li&gt;&lt;/ol&gt;</ns4:addinfo>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_4"/>
    <foaf:depiction rdf:resource="https://mus.list.brc.riken.jp/ja/wp-content/image_s/00869-P-370-01.jpg"/>
    <ns6:brs_geneticBackground rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/geneticBackground_5"/>
    <skos:altLabel xml:lang="en">B6.D2-Tg(GOF18 delta PE/EGFP)48, GOF18-delta PE-GFP line48 transgenic mouse</skos:altLabel>
    <ns6:brs_depositorOrganization xml:lang="ja">大阪府立母子保健総合医療センター研究所</ns6:brs_depositorOrganization>
    <foaf:depiction rdf:resource="http://ja.brc.riken.jp/lab/bpmp/use_photo/mouse/00869.jpg"/>
    <dcterms:isReferencedBy rdf:parseType="Resource">
    </dcterms:isReferencedBy>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainType_24"/>
    <ns7:SIO_001279 rdf:parseType="Resource">
    </ns7:SIO_001279>
    <dc:description xml:lang="en">Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999).  Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages.  This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter.  Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198).</dc:description>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_6"/>
    <ns4:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr00869'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns4:addinfo>
  </rdfs:Class>
  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/Phenotype_1015111">
    <ns6:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC00869"/>
  </rdf:Description>
  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/RecoveryTestResult_203">
    <ns6:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC00869"/>
  </rdf:Description>
</rdf:RDF>