特になし。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 449, 277-283 (1999). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. 開発者:岡部勝先生機関名:大阪大学遺伝情報実験センターBCF1 その後不明。 Carrier x Carrier (Homozygote x Homozygote) Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. Acr3-EGFP transgene was injected into the pronuclei of B6C3F1 fertilized eggs. The mice were crossed to C57BL/6. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。FEBS Lett., 449, 277-283 (1999). <br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 Fluorescent Proteins/lacZ System 大阪大学 B6;B6C3-Tg(Acro3-EGFP)01Osb B6;B6C3-Tg(Acro3-EGFP)01Osb B6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01 B6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01 C (3-6 months) Masaru OKABE RBRC00886 mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, Jellyfish GFP cDNA, rabbit beta-globin polyadenylation signal 精子の先体部分が480 nmで励起すれば光る。 C(3〜6か月) 岡部 勝 Osaka Univ. 開発者:岡部勝先生 機関名:大阪大学遺伝情報実験センター BCF1 その後不明。 true <a href='https://brc.riken.jp/mus/pcr00886'>Genotyping protocol -PCR-</a> Transgenic mice expressing GFP in the sperm acrosome from acr3-EGFP. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter.