京都大学 AID-KO C57BL/6 N10, B6-AID KO#N10, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc AID-KO C57BL/6 N10, B6-AID KO#N10, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc Tasuku HONJO 本庶　佑 Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mutant mice were backcrossed to C57BL/6N. <a href='https://brc.riken.jp/mus/pcr00897'>Genotyping protocol -PCR-</a> TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www2.mfour.med.kyoto-u.ac.jp/en/index.html" target="_blank">Honjo Lab HP</A> Homozygote x Homozygote. Homozygous mutant mice are viable and fertile. SPFであれば、特に飼育、交配は、通常通り可能。 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. <br>2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Cell 102, 553-563 (2000).<br>3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. 条件を付加する。<br>1. 公表を前提とした学術研究に限る。<br>2. 研究成果の公表にあたって寄託者への謝辞の表明並びに寄託者の指定する文献を引用する。Cell 102, 553-563 (2000).<br>3. 本件リソースの改変体又は本件リソースの生産方法若しくは使用方法に係る特許を出願した場合、その旨を速やかに寄託者に通知する。 軽度の液性免疫不全がある。,IgG, IgA, IgEを産生しない。 AID (Aicda, activation-induced cytidine deaminase) knockout mice. Exon 2 and part of exon 3 were replaced with a neomycin resistance cassette. Aid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cell is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene was a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also indicated that somatic hypermutation of an immunoglobulin variable region gene and gene conversion do not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell. phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc Kyoto Univ. Immunology and Inflammation Research true B (1-3 months) 軽度の液性免疫不全がある。IgG, IgA, IgEを産生しない。 RBRC00897 常法にて遺伝子破壊マウスを作る。Genetic backgroundは(C57BL/6 x CBA) x C57BL/6を親として、これに10回C57BL/6へ戻し交配した。Cell, 102, 553-563 (2000).参照 Cre/loxP system <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/dec_2005_mm" target="_blank">Mouse of the Month Dec 2005</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/00897_GB.pdf">Genetic Background</a> B（1〜3か月）