PD-1遺伝子はCD28ファミリーに属する免疫抑制受容体であり，活性化したリンパ球に発現し，リンパ球の機能を抑制する．PD-1 (Pdcd1) 遺伝子をノックアウトしたマウスではドナーに用いたマウスの遺伝的背景によってさまざまな自己免疫疾患を発症する．BALB/c背景のPD-1遺伝子ノックアウトマウスは自己免疫性の拡張型心筋症を発症することが示されている．自己免疫疾患制御のメカニズム，その遺伝的要因の関与の解明に有用． Kyoto Univ. C (3-6 months) Sibling Mating (Homozygote x Homozygote)Homozygous mutant mice are fertile and viable. Developed by Dr. Tasuku Honjo, Kyoto University. Tasuku HONJO RBRC00904 <a href='https://brc.riken.jp/mus/pcr00904'>Genotyping protocol -PCR-</a> 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.<br>2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).<br>3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. 本庶　佑 <A HREF="https://mus.brc.riken.jp/ja/mouse_of_month/mar_2005_mm" target="_blank">Mouse of the Month Mar 2005</A> 文献参照 Immunology and Inflammation Research true C（3〜6か月） N12のライン（BRC No. 00904）はあまり発症がひどくない（ホモ維持可能）。N10のライン（BRC No. 00903）は発症しやすく程度もひどい。 mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA Sibling Mating (Homozygote x Homozygote) Homozygous mutant mice are fertile and viable. PD-1-KO-N12 (BALB/c) PD-1-KO-N12 (BALB/c) 条件を付加する。<br>1. 公表を前提とした学術研究に限る。<br>2. 研究成果の公表にあたって寄託者への謝辞の表明並びに寄託者の指定する文献を引用する。Science 291, 319-322 (2001).<br>3. 本件リソースの改変体又は本件リソースの生産方法若しくは使用方法に係る特許を出願した場合、その旨を速やかに寄託者に通知する。 C.129S2-Pdcd1<tm1Hon>/HonRbrc (N12) C.129S2-Pdcd1<tm1Hon>/HonRbrc (N12) D3 [129S2/SvPas] The PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and the immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line. 京都大学 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www2.mfour.med.kyoto-u.ac.jp/en/index.html" target="_blank">Honjo Lab HP</A>