Developed by Dr. Koichi Honke, Osaka University Medical School in 1999. A targeting vector including pgk-neo was transfected into E14.1 ES cells to disrupt exons 2 and 3 of Cst gene. The Cst knockout mice were backcrossed to C57BL/6N for 6 times. Generation: N5+N1F3 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002). TgH(CSTneo) 01 Developed by Dr. Koichi Honke, Osaka University Medical School in 1999. A targeting vector including pgk-neo was transfected into E14.1 ES cells to disrupt exons 2 and 3 of Cst gene. The Cst knockout mice were backcrossed to C57BL/6N for 6 times. Generation: N5+N1F3 繁殖効率：Aヘテロマウスの交配により維持する。ホモマウスは振せん、運動失調等の神経症状と男性不妊を来たす。 Kochi Univ. Koichi HONKE C (3-6 months) cerebroside sulfotransferase(CST)(EC2.8.2.11)遺伝子を相同組換えにより破壊したマウスで、硫酸化糖脂質の生理機能を調べる為に使用する。 B6;129P2-Gal3st1<tm1Kho>/KhoRbrc 開発年：1999年開発者：本家孝一先生機関名：大阪府立母子保健総合医療センター研究所作出系統C57BL/6、ES細胞株E14.1(129Svj)、C57BL/6へのバッククロス。ESクローンNo.98由来。 true 繁殖効率：A ヘテロマウスの交配により維持する。 ホモマウスは振せん、運動失調等の神経症状と男性不妊を来たす。 mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Gal3st1 genomic DNA <a href='https://brc.riken.jp/mus/pcr00961'>Genotyping protocol -PCR-</a> In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002). 開発年：1999年 開発者：本家孝一先生 機関名：大阪府立母子保健総合医療センター研究所 作出系統C57BL/6、ES細胞株E14.1(129Svj)、C57BL/6へのバッククロス。ESクローンNo.98由来。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> RBRC00961 本家　孝一 B6;129P2-Gal3st1<tm1Kho>. Cerebroside sulfotransferase (Cst) gene is located in the Golgi membrane that is responsible for the biosynthesis of two sulfated glycolipids (GalCer and GalEAG) in the mammals. Cst null mice lack sulfatide in myelin and sulfated seminolipid in sperm. Homozygous mutant males are sterile. This strain is useful for elucidation of the role of sulfoglycolipids in myelin function and spermatogenesis. C57BL/6N x CST KO-1(hetero), TgH(CSTneo) 01 C57BL/6N x CST KO-1(hetero), TgH(CSTneo) 01 C（3〜6か月） C57BL/6N x CST KO-1(hetero) C57BL/6N x CST KO-1(hetero) E14.1 [129P2/OlaHsd] 高知大学