<a href='https://brc.riken.jp/mus/pcr00995'>Genotyping protocol -PCR-</a> 東京大学 東京大学医科学研究所・高津聖志先生(1996)。E14.1 ES細胞を用いて作出。C57BL/6CrSlcへ戻し交配された。 L-5Rα遺伝子のノックアウトマウス。エクソン5にSTOPコドンを挿入、エクソン6をneoカセットで置換。ホモマウスはB-1細胞の減少を呈する。生存性、繁殖性あり。 RBRC00995 Kiyoshi TAKATSU Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> E14.1 [129P2/OlaHsd] 高津 聖志 B6.129P2-Il5ra<tm1Ktk>/KtkRbrc B6.129P2-Il5ra<tm1Ktk>/KtkRbrc In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity 4, 483-494 (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided. <br>Users should contact the depositor in the case of application for any patents with the results from these mice. L-5Rα遺伝子のノックアウトマウス。エクソン5にSTOPコドンを挿入、エクソン6をneoカセットで置換。ホモマウスはB-1細胞の減少を呈する。生存性、繁殖性あり。, C (3-6 months) Developed by Kiyoshi Takatsu, Institute of Medical Science in 1996. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6CrSlc. L-5Ralpha gene knockout mice. A translational stop codon was introduced into exon 5 of the Il5ra and the exon 6 was replaced with a neo cassette. Immunology and Inflammation Research Univ. Tokyo mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse IL-5R α genomic DNA 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Immunity 4, 483-494 (1996). <br>研究成果の公表にあたって謝辞の表明を必要とする。<br>共著を必要とする共同研究は寄託後1年間に限定する。<br>利用者が当該リソースを使用した研究成果に基づく特許等の申請を行う場合は、寄託者と別途協議を行う。 C57BL/6 IL-5R alpha -/- C57BL/6 IL-5R alpha -/- C(3〜6か月) Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] true