The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 519-530 (1997).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.<br>When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration. Semaphorin III/D (Sema3a) knockout mice (lacZ reporter). Most of homozygous mice in C57BL/6 genetic background are lethal. About one-tenth of the homozygous mutant mice of ICR background survived (Depositor, personal communication). Neurobiology Research 岡崎国立共同研究機構 Cre/loxP system Semaphorin III/D (Sema3a) 遺伝子のノックアウトマウス (lacZレポーター) 。C57BL/6背景のホモマウスは出生後、高確率で死亡する。ICR背景ではホモマウスの約10％が生存する (寄託者、私信) 。Nullアレル (RBRC01104) 、lacZアレル (RBRC01105) 、floxedアレル (RBRC01106) 。 <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/aug_2006_mm" target="_blank">Mouse of the Month Aug 2006</A> Heterozygote x Wild-type [or Crossing to C57BL/6NCrlCrlj] Heterozygote x Wild-type [or Crossing to C57BL/6NCrlCrlj] Developed by Masahiko Taniguchi and Takeshi Yagi, National Institute for Physiological Sciences in 1997. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 5 times. C（3〜6か月） 生理学研究所・谷口　雅彦先生、八木　健先生（1997）。TT2 ES細胞を用いて作出。C57BL/6へ戻し交配された（N5以上）。 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Neuron, 19, 519-530 (1997).<br>本件リソースの特許権ならびに知的所有権は寄託者が保持しており、利用者が本件リソースから得られた結果により特許等を申請する場合には、事前に寄託者と協議する。利用者は本件リソースを利用して寄託後2年間以内に行った研究結果等を学術論文発表する場合には寄託者との共著の要否について別途協議する。 B6.Cg-Sema3a<tm1.3Tyag>/TyagRbrc phage P1 loxP sites, E. coli lacZ, SV40 early pA signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse semaphorin 3A genomic DNA TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] C (3-6 months) true RBRC01105 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.fbs.osaka-u.ac.jp/labs/yagi/index.htm" target="_blank">Lab HP (Japanese)</A> semaphorin III/D (lacZ) Targeting mice semaphorin III/D (lacZ) Targeting mice Natl. Inst. Basic Biol.,Okazaki Inst. Integrative Bioscience 八木　健 semaphorin III/D (lacZ) Targeting mice Takeshi YAGI Fluorescent Proteins/lacZ System <a href='https://brc.riken.jp/mus/pcr01105'>Genotyping protocol -PCR-</a>