C (3-6 months) 野田　哲生 Heterozygote x Wild-typeHomozygous mutant males are sterile. C（3〜6か月） B6;129S4-Cnot7<tm1Tno>. Cnot7 gene (CCR4-NOT transcription complex. subunit 7) is essential for spermatogenesis. Cnot7 deficient male mice are sterile owing to oligo-astheno-teratozoospermia, impaired sperm motility, unsynchronized spermatid maturation, and Sertoli cell abnormalities. This knockout mouse strain is a powerful tool for understanding the mechanisms underlying spermatogenesis. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Developed by Drs. Ryoji Yao, Tadashi Yamamoto, and Tetsuo Noda, Japanese Foundation for Cancer Research Cancer Institute in 1999. An exon of Cnot7 subcloned the 18-kb genomic DNA fragment, was replaced by the insertion of a LacZ and a floxed neo cassette. The floxed neo cassette was excised in this strain. This strain originated on a B6;129 background. Heterozygote x Wild-type Homozygous mutant males are sterile. 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nature Genetics, 36, 528-533 (2004).<br>・利用者が本件リソースを利用して発明をした場合には、その発明にかかる権利は原則、寄託者及び利用者の共有とし、各々の持ち分については二者間の協議のうえ合意により決定する。また、当該発明にかかる特許出願にあたっては、本件リソースの開発者である(財)癌研究会・癌研究所　野田　哲生　に事前に連絡し了解を得る。<br>・利用者は、発明にかかる権利の持ち分について寄託者と誠実に協議するものとする。<br>・利用者は寄託者の研究と競合する用途を避けるために、予定の研究概要を寄託者に知らせ、使用許可を得る。<br>・利用者が本件リソース寄託後2年以内に理研BRCより提供を受けて得た研究成果の発表は開発者との共著とする。 B6;129S4-Cnot7<tm1Tno>/Rbrc Cell Biology Research The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Genetics, 36, 528-533 (2004).It is agreed by the parties hereto that any patent or other intellectual property right to any inventions to be made by or derived of the use by the RECIPIENT of the BIOLOGICAL RESOURCE shall be jointly owned by the RECIPIENT and the DEPOSITOR. The share in the ownership on such patents etc. shall be agreed between the RECIPIENT and the DEPOSITOR. In the event that the RECIPIENT desires to file patent applications for such inventions, the RECIPIENT shall obtain a prior written consent of the DEVELOPER, Dr. Tetsuo Noda before the RECIPIENT files such patent applications.<br>The RECIPIENT should negotiate in good faith with the DEPOSITOR, or its representatives, to agree a fair and reasonable consideration to the DEPOSITOR in respect of the contribution to the said intellectual property rights afforded by the provision of the BIOLOGICAL RESOURCE hereunder.<br>The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.<br>The RECIPIENT agrees to include the DEVELOPER, Dr. Tetsuo Noda as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. Cnot7 Knockout mouse <a href='https://brc.riken.jp/mus/pcr01254'>Genotyping protocol -PCR-</a> E. coli neo, Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, mouse Cnot-7 genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Phage P1 loxP Genetics Research 開発年：2000年にヘテロマウスが産まれる。開発者：中村能久(東大医科研)、山本雅(東大医科研)、野田哲生(癌研)。機関名：東京大学医科学研究所と癌研究会癌研究所間での共同開発。ESはJ1。キメラマウスとC57BL/6Jと掛け合わせ、F1で維持。 開発年：2000年にヘテロマウスが産まれる。 開発者：中村能久(東大医科研)、山本雅(東大医科研)、野田哲生(癌研)。 機関名：東京大学医科学研究所と癌研究会癌研究所間での共同開発。 ESはJ1。キメラマウスとC57BL/6Jと掛け合わせ、F1で維持。 true Tetsuo NODA (財)癌研究会癌研究所 Cnot7 Knockout mouse Cnot7 Knockout mouse J1 [129S4/SvJae] RBRC01254 Cancer Inst. JFCR 雄ホモマウスは不妊