Cre/loxP system <a href='https://brc.riken.jp/mus/pcr01267'>Genotyping protocol -PCR-</a> Targeting of Numb: 1997, Olav Zilian, ISREC Targeting of Numblike: 2001,Olav Zilian, ISREC Olav Zilian Olav Zilian yeast FRT (flipase recombination target) sites, phage P1 loxP site, E. coli neo, mouse Numb genomic DNA, mouse Numblike genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter) Compound heterozygous mice are efficient breeders (Numb[loxP / -]; Numblike[loxP / -]). By contrast, mice homozygous for the deletion of Numblike have a small litter size and, hence, reproduce inefficiently. That problem of low breeding efficiency might rely on diminished fertility of he female homozygotes. Compound heterozygous mice are efficient breeders (Numb[loxP / -]; Numblike[loxP / -]). By contrast, mice homozygous for the deletion of Numblike have a small litter size and, hence, reproduce inefficiently. That problem of low breeding efficiency might rely on diminished fertility of he female homozygotes. Targeting of Numb：1997, Olav Zilian, ISRECTargeting of Numblike：2001,Olav Zilian, ISREC RBRC01267 Cell Biology Research Targeting of Numb: 1997, Olav Zilian, ISRECTargeting of Numblike: 2001,Olav Zilian, ISREC true Numb [-/loxP]; Numblike [-/FRTneoRFRTloxP] Numb [-/loxP]; Numblike [-/FRTneoRFRTloxP] 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.<br>研究成果の公表にあたって謝辞の表明を必要とする。This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.<br>1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.<br>2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.<br>3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. C（3〜6か月） Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 129(Cg)-Numb<tm1Zili/tm1.1Zili> Numbl<tm1Zili/tm1.1Zili>/SiecRbrc 129(Cg)-Numb<tm1Zili/tm1.1Zili> Numbl<tm1Zili/tm1.1Zili>/SiecRbrc Targeting of Numb：1997, Olav Zilian, ISREC Targeting of Numblike：2001,Olav Zilian, ISREC Compound heterozygous mice conditionally targeted for Numb and Numblike (loxP) and some of them deleted for the other conditional allele. The selection marker in Numb (loxPneoRloxP) was deleted by Cre recombinase. However, this cassette is still present in the conditionally targeted allele of Numblike in these mice as a cassette flanked with FRT sites (FRTneoRFRT; [loxn eo]). In general, the purpose of the conditional alleles is to analyze the function of the Numb and Numblike proteins in mice after their conditional ablation upon expression of Cre recombinase. Parents heterozygous for a deleted allele of each gene allow to generate (1) compound homozygous null mutant embryos for the study of embryonic phenotypes or (2) adult mice homozygous for the deletion of Numblike. Compound heterozygous mice conditionally targeted for Numb and Numblike (loxP) and some of them deleted for the other conditional allele. The selection marker in Numb (loxPneoRloxP) was deleted by Cre recombinase. However, this cassette is still present in the conditionally targeted allele of Numblike in these mice as a cassette flanked with FRT sites (FRTneoRFRT; [loxn eo]). In general, the purpose of the conditional alleles is to analyze the function of the Numb and Numblike proteins in mice after their conditional ablation upon expression of Cre recombinase. Parents heterozygous for a deleted allele of each gene allow to generate (1) compound homozygous null mutant embryos for the study of embryonic phenotypes or (2) adult mice homozygous for the deletion of Numblike. Institut Suisse de Recherche Experimentale sur le Cancer (ISREC) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.<br>2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.<br>3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. ISREC C (3-6 months)