Targeting of Numb：1997, Olav Zilian, ISRECTargeting of Numblike：2001,Olav Zilian, ISREC Cell Biology Research Targeting of Numb：1997, Olav Zilian, ISREC Targeting of Numblike：2001,Olav Zilian, ISREC C (3-6 months) 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.<br>研究成果の公表にあたって謝辞の表明を必要とする。This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.<br>1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.<br>2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.<br>3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Targeting of Numb: 1997, Olav Zilian, ISREC Targeting of Numblike: 2001,Olav Zilian, ISREC ISREC phage P1 loxP, mouse Numb genomic DNA, mouse Numblike genomic DNA, yeast FRT (flipase recombination target) site Compound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny. Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase. Olav Zilian Olav Zilian The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Any publication by the DEPOSITOR describing the BIOLOGICAL RESOURCE for the first time. (For targeted Numb: Olav Zillan et al. Current Biology. 11(7): 494-501; for targeted Numblike : the first publication describing this allele by the DEPOSITOR). This term is to be respected in particular when the first 2 years after deposition of the BIOLOGICAL RESOURCE are passed.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. This term is to be respected in particular when the material was requested after the first 2 years upon deposition of the BIOLOGICAL RESOURCE.1) The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. Furthermore, the RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE in research projects initiated during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.<br>2) Further, the RECIPIENT must inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.<br>3) Furthermore, prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Cre/loxP system B6;129-Numb<tm1Zili> Numbl<tm1Zili>/SiecRbrc B6;129-Numb<tm1Zili> Numbl<tm1Zili>/SiecRbrc true Compound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny.Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase. Compound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny.Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase. RBRC01268 C（3〜6か月） Numb [loxP/loxP]; Numblike [loxP/loxP] Numb [loxP/loxP]; Numblike [loxP/loxP] Efficient breeding is expected for these compound mice once homozygous for each conditionally targeted allele (Numb[lox / lox]; Numblike[lox / lox]) as they were efficient breeders when the compound homozygotes still carried FRTneoRFRT in both Numblike lox alleles. Efficient breeding is expected for these compound mice once homozygous for each conditionally targeted allele (Numb[lox / lox]; Numblike[lox / lox]) as they were efficient breeders when the compound homozygotes still carried FRTneoRFRT in both Numblike lox alleles. Targeting of Numb: 1997, Olav Zilian, ISRECTargeting of Numblike: 2001,Olav Zilian, ISREC Institut Suisse de Recherche Experimentale sur le Cancer (ISREC) Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> <a href='https://brc.riken.jp/mus/pcr01268'>Genotyping protocol -PCR-</a> Compound mice conditionally targeted for Numb and Numblike (loxP; for nomenclature of the alleles: lox) ; the selection marker in Numb (loxPneoRloxP) and Numblike (FRTneoRFRT) was deleted by Cre and Flp recombinase, respectively. The mice ready for shipment to RIKEN BRC carry at this point the conditional alleles over a non-targeted, wild-type allele [lox/+]; absence of the transgene FLPe should be reconfirmed in their progeny.,Purpose of these conditional alleles: functional analysis of the Numb and Numblike proteins in mice by their conditional ablaion through expression of Cre recombinase.