C（3〜6か月） C (3-6 months) REP-DT REP-DT Osaka Univ. 国立大学法人大阪大学 CAG-DTコンディショナルトランスジェニックマウス (REP-DTマウス) 。creリコンビネース存在下で，loxP-neo-loxP配列が除かれるとCAGプロモーターの制御下にジフテリア毒素Aフラグメント遺伝子が発現する。ジフテリア毒素は細胞の蛋白合成を停止させ、致死させる。精母細胞特異的にジフテリア毒素Aフラグメント遺伝子を発現したマウスでは生殖細胞系列へ障害が確認されている。他の臓器組織での発現パターン等は不明 (トランスジーンが挿入された遺伝子座等の影響により臓器組織によっては発現パターンが左右される可能性がある) 。 true General Purpose B6.Cg-Tg(CAG-floxed neo-DT)03Osb B6.Cg-Tg(CAG-floxed neo-DT)03Osb 岡部　勝 Carrier x Carrier, Noncarrier [or Crossing to C57BL/6NCrSlc] Carrier x Carrier, Noncarrier [or Crossing to C57BL/6NCrSlc] Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, E. coli neo, Corynebacterium diphtheriae diphtheria toxin A fragment DNA, rabbit beta-globin poly A Developed by Masaru Okabe, Genome Information Research Center, Osaka University in 2003. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background. RBRC01347 Cre/loxP system CAG-DT conditional transgenic mice (REP-DT mice). The transgene consists of the CAG promoter and the diphtheria toxin A chain (DT) cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, DT is expressed. It has been reported that healthy but sterile mice due to a disruption of germ line cells are generated when the REP-DT mice were mated with testis specific cre mice (Prm1-cre). Other tissue/organ-specific expression patterns of the transgene are not clear (Caution: The gene expression pattern in transgenic mice can be different from it of the endogenous gene.) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 321, 275-279 (2004).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. Masaru OKABE <a href='https://brc.riken.jp/mus/pcr01347'>Genotyping protocol -PCR-</a> 大阪大学遺伝情報実験センター・岡部　勝先生（2003）。B6D2F1受精卵へトランスジーンをインジェクションすることにより作出。C57BL/6とDBA/2の混合背景。 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophys. Res. Commun., 321, 275-279 (2004).<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること（https://www.ccb.osaka-u.ac.jp/en/）。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。