The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Genetics, 35, 238-245 (2003).The DEPOSITOR should be a co-author in the articles when the users publish their data using these mice for 2 year after deposition to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. B6;129P2-Keap1<tm1Mym>/MymRbrc Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://www.dmbc.med.tohoku.ac.jp/official/index.html" target="_blank">Lab HP (Japanese)</A> 筑波大学　TARA センター　山本プロジェクト Masayuki YAMAMOTO 山本　雅之 E14 [129P2/OlaHsd] true Keap1 knockout mice Simian virus 40 large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Keap1 genomic DNA <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/oct_2005_mm" target="_blank">Mouse of the Month Oct 2005</A> ホモマウスは致死なので、遺伝子型のヘテロのマウスを維持。 Keap1 knockout mice Keap1 knockout mice Developed by Masayuki Yamamoto, Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 2003. Sequence encoding 8 through 204 of Keap1 was replaced with neomycin and nls-lacZ cassette. 国立大学法人筑波大学 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nature Genetics, 35, 238-245 (2003).<br>寄託後2年間は共著を必要とする共同研究とする。当該リソースを使用した研究結果に基づき特許等の申請を行う場合は、寄託者と別途協議を行う。 Univ. Tsukuba C (3-6 months) <a href='https://brc.riken.jp/mus/pcr01388'>Genotyping protocol -PCR-</a> Keap1 gene is a cytoplasmic protein, acts as a negative regulator of Nrf2 and as a sensor of xenobiotic and oxidative stresses. Double knockout mice of Nrf2 and Keap1 demonstrated that Nrf2 is a primary target of Keap1. Nrf2-Keap1 system is one of the major cellular protection mechanisms against xenobiotic and oxidative stresses. Keap1 homozygous mutant mice exhibit scaly skin, hyperkeratosis in the esophague and forestomatch, and died postnatally. Heterozygote x Wild-type [C57BL/6JJcl]. Homozygous mutant mice are postnatal letha. RBRC01388 C（3〜6か月） Fluorescent Proteins/lacZ System