The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediates to catalyze xenobiotic Phase I metabolism. Human AhR knock in mouse, possessing human AHR instead of mouse Ahr, is useful as humanized mouse to predict the biological effects of bioaccumulative environmental toxicants like TCDD in humans. Univ. Tsukuba C (3-6 months) 2001 年　森口尚先生、筑波大学 Developed by Dr. Takashi Moriguchi et al. in 2001. Human AHR cDNA and neomycin resistant gene were inserted in mouse Ahr gene. This construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. This knock-in mice were backcrossed with C57BL/6J for several times. C（3〜6か月） RBRC01389 Backcross to C57BL/6 (Heterozyote x C57BL/6JJ) Backcross to C57BL/6 (Heterozyote x C57BL/6JJ) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. 国立大学法人筑波大学 <a href='https://brc.riken.jp/mus/pcr01389'>Genotyping protocol -PCR-</a> human AhR がノックインされたマウス 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>寄託後2年間は共著を必要とする共同研究とする。当該リソースを使用した研究結果に基づき特許等の申請を行う場合は、寄託者と別途協議を行う。 human arylhydrocarbon receptor AhR cDNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse arylhydrocarbon receptor AhR genomic DNA 山本　雅之 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://www.dmbc.med.tohoku.ac.jp/official/index.html" target="_blank">Lab HP (Japanese)</A> true B6;129P2-Ahr<tm1(AHR)Mym>/MymRbrc B6;129P2-Ahr<tm1(AHR)Mym>/MymRbrc E14 [129P2/OlaHsd] Human AhR knock in mouse Human AhR knock in mouse Masayuki YAMAMOTO