<a href='https://brc.riken.jp/mus/pcr01390'>Genotyping protocol -PCR-</a> B (1-3 months) Nrf2 (Nfe2l2) Knockout mice. This strain is a useful model for the in vivo analysis of chemical carcinogenesis and resistance to anti-cancer drugs. A phenolic antioxidant significantly induced the expression of phase II enzymes such as glutathione S-transferase and NAD(P)H: quinone oxidoreductase in normal mice. However, in the homozygous Nrf2 deficient mice the induction of the phase II enzymes by a phenolic antioxidant was largely eliminated in the liver and intestine. Since Nrf2 is constantly degraded by proteasome under normal condition, it is hardly to detect Nrf2 protein in the absence of stimuli. Electrophilic chemicals, such as DEM (diethyl maleate) and tBHQ (tert-buthyl hydroquinone), are generally used to stabilize Nrf2. Or proteasomal inhibitors, such as MG132, are also effective increasing the protein amount of Nrf2. C57BL/6 background (RBRC01390), BALB/c background (RBRC02190), A/J background (RBRC02414), ICR mixed background (RBRC00984). Homozygous mutant mice are viable and fertile, but show lower reproductive performance. 筑波大学先端学際領域研究センター・山本雅之先生（1996）。 Univ. Tsukuba B（1〜3か月） B6.129P2-Nfe2l2<tm1Mym>/MymRbrc B6.129P2-Nfe2l2<tm1Mym>/MymRbrc The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 236, 313-322 (1997).The RECIPIENT agrees that it will acknowledge the DEPOSITOR as academically and scientifically appropriate in its articles when the Recipient publishes its data using these mice. Recipient should contact the DEPOSITOR in the case of application for any patents with the results from these mice. true Masayuki YAMAMOTO Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Nrf2 genomic DNA Homozygote x Heterozygote [or Crossing to C57BL/6JJcl] Homozygote x Heterozygote [or Crossing to C57BL/6JJcl] E14 [129P2/OlaHsd] 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Biochem. Biophys. Res. Commun., 236, 313-322 (1997).<br>当該リソースを使用した研究結果に基づき特許等の申請を行う場合は、寄託者と別途協議を行う。 RBRC01390 Nrf2 knockout mouse/C57BL6J Nrf2 knockout mouse/C57BL6J 国立大学法人筑波大学 Fluorescent Proteins/lacZ System Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://www.dmbc.med.tohoku.ac.jp/official/index.html" target="_blank">Lab HP (Japanese)</A> Developed by Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 1996. The targeting vector containing lacZ-neo cassette was transferred into E14 ES cells to replace the 1.2 kb segment containing the rest of the exon 5 coding sequence of the Nrf2 gene. <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/oct_2005_mm" target="_blank">Mouse of the Month Oct 2005</A><br><A HREF="https://mus.brc.riken.jp/en/mouse_of_month/apr_2012_mm" target="_blank">Mouse of the Month Apr 2012</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/01390_GB.pdf">Genetic Background</a><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/01390_BLC.pdf">Breeding characters</a> 山本　雅之 Nrf2 (Nfe2l2) 遺伝子のノックアウトマウス。Nrf2は生体防御システムの遺伝子群の発現を活性化させる転写因子として知られている。活性酸素種、親電子性物質、重金属などの生体異物にさらされると、それらの毒性を排除するために解毒化酵素や酸化ストレス応答蛋白質が転写誘導される。Nrf2はそれらの転写の発現を誘導している。Nrf2ノックアウトマウスでは、抗酸化剤応答配列を介した遺伝子発現が減弱しており、親電子物質に対して感受性が高くなっている。また、親電子性物質が遺伝子点変異を誘起することから、Nrf2遺伝子が発ガンや動脈硬化、脳神経障害の予防に役立つ可能性も期待されている。C57BL/6背景 (RBRC01390) 、BALB/c背景 (RBRC02190) 、A/J背景 (RBRC02414) 、ICR混合背景 (RBRC00984) 。ホモマウスは生存性、繁殖性はあるものの、繁殖効率は低くなる。