Developed by Dr. Kenjiro Inagaki, Kobe University School of Medicine in 2000. The construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed C57BL/6N over 10 times. Heterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile. true E14.1 [129P2/OlaHsd] C (3-6 months) 繁殖効率：B 野生型に比べ、繁殖効率が劣る印象、定量的データはなし。 繁殖成績が低い SHPS-1 Knockout mice SHPS-1 Knockout mice Takashi MATOZAKI SHPS-1 knockout mice. Exon 7 and 8 were replaced with a nemoycin selection cassette. SHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene ia a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.imcr.gunma-u.ac.jp/gcoe/eng/organization/members/matozaki/index.html" target="_blank">Lab HP</A> 129/Sv 系統由来 ES 細胞 (E14.1) にターゲティングベクターを導入し作製。膜タンパク質SHPS-1 の機能解析に使用。 繁殖効率：B野生型に比べ、繁殖効率が劣る印象、定量的データはなし。繁殖成績が低い 国立大学法人群馬大学 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must submit its receipt to the DEPOSITOR immediately after receiving the BIOLOGICAL RESOURCE. <a href='https://brc.riken.jp/mus/pcr01544'>Genotyping protocol -PCR-</a> 的崎　尚 <a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/01544_GB.pdf">Genetic Background</a> RBRC01544 神戸大学で作製。10世代　C57BL/6Nにバッククロス。 C（3〜6か月） 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>群馬大学生体調節研究所に受領書を提出する。 E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse Sirpa genomic DNA Gunma Univ. B6.129P2-Sirpa<tm1Nog>/Rbrc B6.129P2-Sirpa<tm1Nog>/Rbrc