TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] C（3〜6か月） Developed by Dr. Kadowaki of the University of Tokyo and backcrossed to C57BL/6 for 12 generations by depositor. 小安　重夫 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. PI3K, phosphoinositide-3-kinase gene is a lipid kinases that catalyze the addition of a phosphate molecule specifically to the 3-position of the inositol ring of phosphoinositides. This strain is useful for elucidating of the rule of PI3K in various phases of immune responses. Homozygous mutant females were sterile. Since most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males. Since most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males. C57BL/6-PI3K KO C57BL/6-PI3K KO Shigeo KOYASU 慶應義塾大学 <a href='https://brc.riken.jp/mus/pcr01578'>Genotyping protocol -PCR-</a> true C (3-6 months) B6.Cg-Pik3r1<tm1Tka>/Rbrc B6.Cg-Pik3r1<tm1Tka>/Rbrc 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).<br>研究成果の公表にあたって謝辞の表明を必要とする。 E. coli neo, HSV TK polyA, mouse PI3K genomic DNA RBRC01578 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.ims.riken.jp/labo/43/index.html" target="_blank">Lab HP</A> Keio University 1999年に東大の門脇 孝先生と共同で作成し、その後 C57BL/6NTac と BALB/cATac に12代戻し交配したもの