開発年：2005年開発者：竹田潤二先生機関名：大阪大学先端科学イノベーションセンター　バイオ・生命系分野Mixed background Developed by Dr. Junji Takeda at Osaka University in 2005. true 国立大学法人大阪大学 PV384 開発年：2005年 開発者：竹田潤二先生 機関名：大阪大学先端科学イノベーションセンター　バイオ・生命系分野 Mixed background Fluorescent Proteins/lacZ System C (3-6 months) Junji TAKEDA Gene trap 型の Sleeping Beauty transposon vector の germline での転移によって遺伝子変異を導入。Transposon vector については、参考文献を参照。 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Methods., 2, 763-769 (2005).<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。 繁殖効率：A Gene trap 型の Sleeping Beauty transposon vector の germline での転移によって遺伝子変異を導入。,Transposon vector については、参考文献を参照。 Gene trap of Smoc1 (SPARC related modular calcium binding 1), PV384 Gene trap of Smoc1 (SPARC related modular calcium binding 1), PV384 RBRC01680 Salmon Sleeping Beauty transposon, Salmon Sleeping Beauty transposase, Jellyfish GFP gene, E. coli LacZ gene, E. coli neo gene, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human bcl-2 splice acceptor, Mouse hprt splice donor, Cytomegalovirus enhancer, Chicken beta-actin promoter, Mouse pgk promoter, Rabbit beta-globin polyadenylation signal sequence, Mouse synaptonemal protein 1 promoter, Human growth hormone polyadenylation signal sequence, pBluescript B6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. <a href='https://brc.riken.jp/mus/pcr01680'>Genotyping protocol -PCR-</a> C（3〜6か月） Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://www.med.osaka-u.ac.jp/pub/mr-envi/www/english/index.html" target="_blank">Takeda Lab HP</A> Osaka Univ. 竹田　潤二 Gene trap of Smoc1 (SPARC related modular calcium binding 1) Gene trap of Smoc1 (SPARC related modular calcium binding 1) This strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system. Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)