Tisp40 KO mouse Tisp40 KO mouse Heterozygote x Homozygote [or Crossing to C57BL/6NCrSlc] Heterozygote x Homozygote [or Crossing to C57BL/6NCrSlc] Cell Biology Research 国立大学法人大阪大学 C(3〜6か月) TISP40 (Creb3l4) 遺伝子のノックアウトマウス。エクソン5から10をneoカセットで置換。TISP40はマウス精子形成過程に特異的に転写誘導される遺伝子であり、Histone置換への関与が示唆されている。生体内におけるTISP40遺伝子の機能解析に有用。ホモマウスは繁殖性、妊性あり。 <a href='https://brc.riken.jp/mus/pcr01942'>Genotyping protocol -PCR-</a> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Genes to Cells, 11, 1161-1171 (2006).<br>共同研究 TISP40 (Creb3l4) gene knockout mice. Exons 5 to 10 were replaced with a neo cassette. Homozygous mutant mice are viable and fertile. Osaka Univ. RBRC01942 true Reproductive Biology Research The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes to Cells, 11, 1161-1171 (2006).The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. C (3-6 months) Hiroshi NOJIMA B6;129S2-Creb3l4<tm1Noj> B6;129S2-Creb3l4<tm1Noj> Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 大阪大学微生物病研究所・薮田紀一先生、野島 博先生(2004)。D3 ES細胞を用いて作出。C57BL/6へ戻し交配(N6以上)。 D3 [129S2/SvPas] mouse Creb3l4 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo 野島 博 Developed by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2004. D3 ES cells were used. The mutant mice were crossed to C57BL/6 over 6 times.