条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Development, 133, 4925-4931 (2006).<br>マウスを使用するに当たり、寄託者を競合する研究でないことを確認する。成果によるパテント等の申請は寄託者と別途協議する。 J1 [129S4/SvJae] Fluorescent Proteins/lacZ System C (3-6 months) RBRC01949 C（3〜6か月） Tsix SA; Tsix splicing defect mutant mice Tsix SA; Tsix splicing defect mutant mice In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 133, 4925-4931 (2006).The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.<br>The RECIPIENT should contact the DEPOSITOR in the case of application for any patents with the results from these mice. Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Phage P1 loxP, mouse Tsix genomic DNA Takashi SADO <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/feb_2009_mm" target="_blank">Mouse of the Month Feb 2009</A> 国立遺伝学研究所・佐渡　敬先生（2006）。J1 ES細胞を用いて作出。 true Developed by Takashi Sado, National Institute of Genetics in 2006. J1 ES cells were used to generate the mutant mice. Puromycin resistance was excised by crossing with cre transgenic mice. Res. Org. Info. System B6;129S4(Cg)-Tsix<tm1Sado> B6;129S4(Cg)-Tsix<tm1Sado> Tsix遺伝子のスプライシングノックアウトマウス (Tsix<SA>) 。TsixはX染色体不活性化に必須であるXist遺伝子のアンチセンス遺伝子。Tsix RNAの主要なスプライシング産物の産出を阻害。父親由来のmutant alleleをもつ雌は胚性致死。Xistノックアウトマウス同様、mutantアレルをもつオス親から受け継いだ場合、メスは全て致死となる。しかし、ヘテロのメスから受け継いだ場合はXistノックアウトマウス同様、ミュータントマウスは次世代へ伝播することができる。Tsix遺伝子ノックアウト (Xist<1loxGFP>) (RBRC01949) 、Tsix遺伝子スプライシングノックアウト (Tsix<SA>) (RBRC01949) 、Xist/Tsixダブルノックアウト (X<dc>) (RBRC01950) 、Tsix<pA> (RBRC02653) 、Xist<IVS> (02654) 、Xist<delta> (RBRC02655) 。 佐渡　敬 Tsix gene splicing knockout mice (Tsix<SA>). The splicing acceptor site of Tsix exon 4 was replaced with a fragment containing an IRES-EGFP and a floxed puromycin resistance gene. This mutant line carries modified allele of Tsix locus to avoid the production major splicing variant. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655). Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://www.bioreg.kyushu-u.ac.jp/labo/epigenome/index.html" target="_blank">Lab HP (Japanese)</A> <a href='https://brc.riken.jp/mus/pcr01949'>Genotyping protocol -PCR-</a> 大学共同利用機関法人　情報・システム研究機構国立遺伝学研究所