<a href='https://brc.riken.jp/mus/pcr02240'>Genotyping protocol -PCR-</a> STOCK Grid2<tm1Mim> Tg(Pcp2-Grid2*R514K)1Yuza STOCK Grid2<tm1Mim> Tg(Pcp2-Grid2*R514K)1Yuza 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>GluRd2KOマウスの使用に際しては、GluRδ2KO mouse（BRC No.01657）の下記の寄託条件の範囲内で利用する。「寄託者の提供承諾書を得る。研究成果の公表にあたって謝辞の表明を必要とする。利用者が本リソースを用いて得た成果に基づき特許、知的所有権、または他の権利を申請する場合には、寄託者の同意を必要とする。本リソースの使用は共同研究のみに限定する。寄託後2年以内の論文発表あるいは寄託後2年以内に論文発表が無い場合はその後の論文発表の際には、利用者は寄託者を共著者とする必要がある。」 柚崎　通介 In 2001, transgenic mice (Tg(PCP2-Grid2R/K)) lines were established by injection into fertilized FVB/N mouse eggs by the donating investigator at St. Jude Children's Hospital.In 2002, the transgenic mice were bred on GluR 2-/- background by crossing with B6-Grid2<tm3Mish> lines (RIKEN BRC No.01657). The transgene (Grid2R/K), which has a mutation in the putative ligand binding site of the δ2 glutamate receptor, is expressed specifically in cerebellar Purkinje cells by a PCP2 promoter. The transgene (Grid2R/K), which has a mutation in the putative ligand binding site of the δ2 glutamate receptor, is expressed specifically in cerebellar Purkinje cells by a PCP2 promoter. 慶應義塾大学 Neurobiology Research In 2001, transgenic mice (Tg(PCP2-Grid2R/K)) lines were established by injection into fertilized FVB/N mouse eggs by the donating investigator at St. Jude Children’s Hospital. In 2002, the transgenic mice were bred on GluRδ2-/- background by crossing with B6-Grid2tm3Mish lines (RIKEN BRC No.01657). C (3-6 months) In 2001, transgenic mice (Tg(PCP2-Grid2R/K)) lines were established by injection into fertilized FVB/N mouse eggs by the donating investigator at St. Jude Children's Hospital. In 2002, the transgenic mice were bred on GluR 2-/- background by crossing with B6-Grid2<tm3Mish> lines (RIKEN BRC No.01657). Keio University mouse PCP2 promoter, mouse Grid2 cDNA The mice are supplied as homozygotes for the transgene (Tg(PCP2-Grid2R/K)) on GluRδ2-/- background. Therefore, when crossed within a colony, no genotypings are necessary. The mice are supplied as homozygotes for the transgene (Tg(PCP2-Grid2R/K)) on GluRδ2-/- background. Therefore, when crossed within a colony, no genotypings are necessary. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://www.yuzaki-lab.org/" target="_blank">Lab HP (Japanese)</A> RBRC02240 C（3〜6か月） The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the GluR 2KO mouse (BRC No. 01657) complying with the following terms and conditions requested by the DEPOSITOR of the GluR 2KO mouse "The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for first publication if no publication is made within the TWO years." In 2001, transgenic mice (Tg(PCP2-Grid2R/K)) lines were established by injection into fertilized FVB/N mouse eggs by the donating investigator at St. Jude Children’s Hospital.In 2002, the transgenic mice were bred on GluRδ2-/- background by crossing with B6-Grid2tm3Mish lines (RIKEN BRC No.01657). 繁殖効率：A The mice are supplied as homozygotes for the transgene (Tg(PCP2-Grid2R/K)) on GluRδ2-/- background. Therefore, when crossed within a colony, no genotypings are necessary. true 繁殖効率：AThe mice are supplied as homozygotes for the transgene (Tg(PCP2-Grid2R/K)) on GluRδ2-/- background. Therefore, when crossed within a colony, no genotypings are necessary. GluRδ2 rescue line-R/K GluRδ2 rescue line-R/K Michisuke YUZAKI