iPS細胞 (誘導性多能性幹細胞 induced pluripotent stem cell) 作製系統 (Nanog-GFPレポーター系統) 。Tgホモ/ヘミマウスともに生存性、繁殖性あり。性差の偏り (雄の偏り) あり (詳細不明) 。 C（3〜6か月） Fluorescent Proteins/lacZ System Developmental Biology Research true RF8 [129S4/SvJae] C (3-6 months) <a href='https://brc.riken.jp/mus/pcr02290'>Genotyping protocol -PCR-</a> RBRC02290 Jellyfish EGFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), S. alboniger puromycin resistance gene, bovine growth hormone polyadenylation signal, yeast FRT (flipase recombination target) site, mouse genomic DNA from RP23-117GFPNF STOCK Tg(Nanog-GFP,Puro)1Yam STOCK Tg(Nanog-GFP,Puro)1Yam Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/02290_form_5.docx">Japanese</A>/ <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/02290_form_C.docx">English</A>)</li>Distribution of the iPS cells (<A HREF="https://www.cira.kyoto-u.ac.jp/j/research/material_1.html" target="_blank">Japanese</A>/ <A HREF="https://www.cira.kyoto-u.ac.jp/e/research/material_1.html" target="_blank">English</A>)<br><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> Developed by Dr. Shinya Yamanaka, Institute for Frontier Medical Sciences, Kyoto University in 2003. The construct was injected into RF8 ES cells derived from 129S4/SvJae. 129, C57BL/6 and DBA/2 mixed background. 山中　伸弥 Nanog-GFP transgenic mice. Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). This Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror) cassette into the 5' untranslated region of a bacterial artificial chromosome (BAC) containing the mouse Nanog gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency. Tg homozygous and hemizygous mice were viable and fertile. Male-biased sex ratios was observed (199 females : 261 males)(details unknown). Carrier x Carrier (Homozygote x Homozygote) Carrier x Carrier (Homozygote x Homozygote) Kyoto Univ. FLP/frt system RF8(129S4/SvJae由来)ES細胞をC57BL/6JJclのblastocystにインジェクションして作製。C57BL/6Jに数回戻し交配をした後、DBA/2JJclと1回交配を行い、その後、兄妹交配にて維持。 Cell Biology Research 国立大学法人京都大学 Shinya YAMANAKA <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/dec_2007_mm" target="_blank">Mouse of the Month Dec 2007</A><br><A HREF="https://mus.brc.riken.jp/en/mouse_of_month/oct_2012_mm" target="_blank">Mouse of the Month Oct 2012</A>