RBRC02393 true Aid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency causes a complete defect in class switching and shows a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cells is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene is a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also found that somatic hypermutation of an immunoglobulin variable region gene and gene conversion did not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www2.mfour.med.kyoto-u.ac.jp/en/index.html" target="_blank">Honjo Lab HP</A> Homozyogte x HomozygoteHomozygous mutant mice are viable and fertile. 本庶　佑 兄妹交配（ホモ欠損マウス同士の交配） B（1〜3か月） Tasuku HONJO BALB/c-Aicda<-/->(N12)mouse BALB/c-Aicda<-/->(N12)mouse 条件を付加する。<br>1. 公表を前提とした学術研究に限る。<br>2. 研究成果の公表にあたって寄託者への謝辞の表明並びに寄託者の指定する文献を引用する。Science, 298, 1424-1427 (2002).<br>3. 本件リソースの改変体又は本件リソースの生産方法若しくは使用方法に係る特許を出願した場合、その旨を速やかに寄託者に通知する。 <a href='https://brc.riken.jp/mus/pcr02393'>Genotyping protocol -PCR-</a> Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA [Update June, 4, 2015] Developed by Dr. Masamichi Muramatsu at Kyoto University in 2000. A neomycin resistance cassette was transferred into TT2 ES cells to replace exon 2 and part of exon 3 of the Aicda gene. The Aid deficient mice were backcrossed to BALB/c. B (1-3 months) Cre/loxP system AID-KO (BALB/c 10回backcross #00901)と同じ軽度の液性免疫不全がある。,IgG, IgA, IgEを産生しない。 C.Cg-Aicda<tm1Hon>(N12) C.Cg-Aicda<tm1Hon>(N12) Immunology and Inflammation Research TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] AID-KO (BALB/c 10回backcross #00901)と同じ軽度の液性免疫不全がある。IgG, IgA, IgEを産生しない。 Genetic backgroundは(C57BL/6 x CBA) x C57BL/6を親として、これに12回BALB/cへ戻し交配した。Cell, 102, 553-563 (2000) 参照 Homozyogte x Homozygote Homozygous mutant mice are viable and fertile. 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.<br>2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science, 298, 1424-1427 (2002).<br>3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.