PRAT4A<-/->は殆どのTLRリガンドに対して低応答性 Heterozygote x Wild-type [C57BL/6CrSlc] The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 204, 2963-2976 (2007).Transfer of the BIOLOGICAL RESOURCE to the third party from the RECIPIENT will never be done without a prior permission of the DEPOSITOR. <br>The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE. <br>The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 兄妹交配：ヘテロどうしでの交配致死性：ヘテロどうしの掛け合わせで、+/+ : +/- : -/- = 1: 2 : 0.4程度行動の異常：震癬、尾懸垂試験異常、音反応性癲癇様発作形態の異常／奇形；同腹ヘテロに比べて体重が25-30%程度繁殖障害：ホモでの系統維持は不可能その他：生まれてきた-/-マウスも4週齢までに半数が致死、-/-発育には要離乳食、同腹ヘテロ、野生型との引き離しが必要 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.ims.u-tokyo.ac.jp/kanseniden/index.html" target="_blank">Lab HP (Japanese)</A> 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Exp. Med., 204, 2963-2976 (2007).<br>第三者への分与禁止<br>利用者は使用目的を寄託者に通知<br>研究成果に基づいた特許等の申請は寄託者と別途協議 開発者／機関：三宅健介先生／東京大学医科学研究所開発年：2007年 mouse PRAT4A genomic DNA, mouse PGK promoter, E. coli neo RBRC02399 開発者／機関：三宅健介先生／東京大学医科学研究所 開発年：2007年 兄妹交配：ヘテロどうしでの交配 致死性：ヘテロどうしの掛け合わせで、+/+ : +/- : -/- = 1: 2 : 0.4程度 行動の異常：震癬、尾懸垂試験異常、音反応性癲癇様発作 形態の異常／奇形；同腹ヘテロに比べて体重が25-30%程度 繁殖障害：ホモでの系統維持は不可能 その他：生まれてきた-/-マウスも4週齢までに半数が致死、-/-発育には要離乳食、同腹ヘテロ、野生型との引き離しが必要 <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/apr_2008_mm" target="_blank">Mouse of the Month Apr 2008</A> C57BL/6-PRAT4A<tm1Kmiy> C57BL/6-PRAT4A<tm1Kmiy> C57BL/6-PRAT4A<-/-> C57BL/6-PRAT4A<-/-> 三宅　健介 C（3〜6か月） <a href='https://brc.riken.jp/mus/pcr02399'>Genotyping protocol -PCR-</a> Immunology and Inflammation Research true Developed by Dr. Kensuke Miyake, The University of Tokyo in 2000. Kensuke MIYAKE C57BL/6-PRAT4A<tm1Kmiy>. Immune cells such as dendric cells (DCs) and macrophages express multiple Toll-like receptors (TLRs) which recognize a variety of pathogen products. A protein associated with TLR4 (PRAT4A) has been found to bind TLR4, and regulate the expression of TLR4 and the response to LPS. Using PRAT4A-deficient mice, there are defective cytokine production against bacteria and activation of Th1 responses. DCs, macrophages and B cells lacking PRAT4A have impaired responses to a variety of TLR ligands. The PRAT4A-deficient bone marrow chimera mice are resistant to lethal-dose LPS administration in the LPS-induced sepsis model to analyze in vivo immune response. This mutant mouse is useful for the study of PRAT4A in innate and adaptive immunity. C (3-6 months)