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    xmlns:dcterms="http://purl.org/dc/terms/"
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    xmlns:ns13="http://mus.brc.riken.jp/DataSheet/"
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  <rdfs:Class rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC02417">
    <ns7:SIO_001279 rdf:parseType="Resource">
    </ns7:SIO_001279>
    <ns1:colonyMaintenance xml:lang="ja">兄妹交配 戻し交配 Background系統：C57BL/6J，日本クレア</ns1:colonyMaintenance>
    <skos:altLabel xml:lang="en">β-catenin/Tcf reporter mice</skos:altLabel>
    <skos:altLabel xml:lang="ja">β-catenin/Tcf reporter mice</skos:altLabel>
    <ns6:brs_depositor xml:lang="ja">山田　泰広</ns6:brs_depositor>
    <dc:description xml:lang="ja">マウス個体、臓器、組織における β-catenin/Tcf 転写活性の強度を GFP 量として測定できる。</dc:description>
    <ns4:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Approval form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;GFP Transfer License (&lt;A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude"&gt;English&lt;/A&gt;)&lt;br&gt;Please fill in the &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc"&gt;Schedule A&lt;/A&gt;, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read &lt;A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"&gt; Schedule B&lt;/A&gt;. &lt;/li&gt;&lt;/ol&gt;</ns4:addinfo>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/17483318"/>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/17483318"/>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns1:isRecombinant rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean"
    >true</ns1:isRecombinant>
    <ns1:strainDevelopment xml:lang="ja">開発者／機関：山田泰広先生（岐阜大学） 開発年：2007 C57BL受精卵にトランスジーンを挿入して作出。C57BL/6Jに戻し交配を行う。その後，兄妹交配にて維持。</ns1:strainDevelopment>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns9:RO_0002162 rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10090"/>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/straintype_2"/>
    <ns1:strainDevelopment xml:lang="en">Developed by Yasuhiro Yamada, Gifu University Graduate School of Medicine in 2007.  The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.  Line 392 (main line) (RBRC02415), line 622 (rbrc02416) and line 628 (RBRC02417).</ns1:strainDevelopment>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/18204079"/>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_4"/>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_8"/>
    <ns6:brs_geneticBackground rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/geneticBackground_5"/>
    <ns4:application>Cell Biology Research</ns4:application>
    <rdfs:subClassOf rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/Strain"/>
    <dcterms:identifier>RBRC02417</dcterms:identifier>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns4:termsAndConditions xml:lang="ja">条件を付加する。利用者は事前に寄託者の提供承諾書を得る。&lt;br&gt;寄託者（開発者）の承諾を得る。学術研究に限る。</ns4:termsAndConditions>
    <ns4:application>Fluorescent Proteins/lacZ System</ns4:application>
    <ns6:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_2"/>
    <rdfs:label xml:lang="en">C57BL/6-Tg(TRE-GFP)628Yasu</rdfs:label>
    <rdfs:label>C57BL/6-Tg(TRE-GFP)628Yasu</rdfs:label>
    <ns4:application>Tet system</ns4:application>
    <ns4:application>General Purpose</ns4:application>
    <dc:description xml:lang="en">Beta-catenin/Tcf reporter mice.  These mice express GFP gene under the control of the Tcf/Lef binding sites and a minimal thymidine kinase promoter from the TOPFLASH plasmid.  Immunohistochemistry with a GFP antibody can be used to assess the Tg expression in the intestine.  In the splenocytes, expression of GFP was observed by FACS analysis, In other organs, the GFP expression patterns are not examined yet.</dc:description>
    <ns1:colonyMaintenance xml:lang="ja">兄妹交配戻し交配 Background系統：C57BL/6J，日本クレア</ns1:colonyMaintenance>
    <ns4:application>Developmental Biology Research</ns4:application>
    <ns6:brs_depositor xml:lang="en">Yasuhiro YAMADA</ns6:brs_depositor>
    <ns6:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns6:brs_hasGenomicFeature>
    <ns4:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr02417'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns4:addinfo>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns1:donorDNA>Tcf/Lef binding site  (TOPFLASH plasmid, upstate社、人工的？), TK promoter (HSV), EGFP (jellyfish), intron (SV40), polyA (SV40)</ns1:donorDNA>
    <ns1:strainDevelopment xml:lang="ja">開発者／機関：山田泰広先生（岐阜大学）開発年：2007C57BL受精卵にトランスジーンを挿入して作出。C57BL/6Jに戻し交配を行う。その後，兄妹交配にて維持。</ns1:strainDevelopment>
    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainType_24"/>
    <ns4:resourceTypeLink rdf:resource="http://metadb.riken.jp/terms/xsearch#resource_type_transgene"/>
    <ns6:brs_hasAllele rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/brc_allele515"/>
    <ns1:genotypingProtocol rdf:resource="http://mus.brc.riken.jp/DataSheet/pcr02417"/>
    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/animal/detail.php?brc_no=RBRC02417"/>
    <ns6:brs_depositedBy rdf:parseType="Resource">
    </ns6:brs_depositedBy>
    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
    <ns4:termsAndConditions xml:lang="en">The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.  The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.</ns4:termsAndConditions>
    <ns5:organism rdf:parseType="Resource">
    </ns5:organism>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/18204079"/>
    <ns4:application>Cancer Research</ns4:application>
  </rdfs:Class>
  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/Phenotype_1019855">
    <ns6:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC02417"/>
  </rdf:Description>
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