In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mech Dev., 125, 432-440 (2008). RBRC02492 BAC transgenic, Mesp2 遺伝子座を欠損し、Mesp1の体節での発現を失ったBAC constructをMesp1, Mesp2のダブルノックアウトマウス (ヘテロ) に導入したマウス。BAC由来のMesp1は初期の中胚葉でのみ発現する. <a href='https://brc.riken.jp/mus/pcr02492'>Genotyping protocol -PCR-</a> 2007年、相賀裕美子先生、国立遺伝研 C (3-6 months) TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] Developmental Biology Research Yumiko SAGA C（3〜6か月） human alkaline phosphatase-pA cDNA, yeast Frt, BAC(Bacterial artificial chromosome)clone containing mouse Mesp1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo ヘテロで維持 BAC- Delta P2 Delta EME#2/Mesp1 Mesp2-dko ICR.Cg-Mesp1/Mesp2<tm1Ysa>Tg(deltaP2deltaEME)2Ysa/YsaRbrc ICR.Cg-Mesp1/Mesp2<tm1Ysa>Tg(deltaP2deltaEME)2Ysa/YsaRbrc 相賀　裕美子 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.nig.ac.jp/nig/research/organization-top/organization/saga" target="_blank">Saga Lab HP</A> true BAC-ΔP2ΔEME#2/Mesp1 Mesp2-dko 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Mech Dev., 125, 432-440 (2008).