西原 真杉
No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
開発者/機関:東京大学大学院農学生命科学研究科獣医生理学教室および東京大学医科学研究所ヒト疾患モデル研究センターとの共同研究により作出開発年:2001年
Masugi NISHIHARA
20alpha-HSD knock-out mouse
IVCSを背景に20alpha-HSD遺伝子を欠損したマウス
戻し交配(C57BL/6jに7回戻し交配後、IVCSに12回戻し交配)
Fluorescent Proteins/lacZ System
IVCS.129P2(B6)-Akr1c18<tm1Mni>
IVCS.129P2(B6)-Akr1c18<tm1Mni>
20a-HSD IVCS
Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish EGFP cDNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine Growth Hormone poly-A signal,mouse 20 alpha-hydroxysteroid dehydrogenase genomic DNA
E14.1 [129P2/OlaHsd]
条件を付加しない。
RBRC02599
true
開発者/機関:東京大学大学院農学生命科学研究科獣医生理学教室および東京大学医科学研究所ヒト疾患モデル研究センターとの共同研究により作出 開発年:2001年
C(3〜6か月)
Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol>
C (3-6 months)
<a href='https://brc.riken.jp/mus/pcr02599'>Genotyping protocol -PCR-</a>
Reproductive Biology Research