条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>寄託者（開発者）の承諾を得る。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/feb_2009_mm" target="_blank">Mouse of the Month Feb 2009</A> B6;129-Xist<tm5Sado> B6;129-Xist<tm5Sado> 国立遺伝学研究所・佐渡　敬先生（2007）。R1 ES細胞を用いて作出。creマウス（B6）との交配によりneo遺伝子は除去。 Xist<delta>マウス。Xist遺伝子第1エクソン内のAリピートと呼ばれる領域を欠失する。mutantアレルを♂親から受け継いだ場合、全ての仔のメス (ヘテロ型) は致死となる。しかし、ヘテロのメスから受け継いだ場合は次世代へ伝播することができる。, R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] RBRC02655 B (1-3 months) Developmental Biology Research Cre/loxP system Xist<delta> mice. The portion of exon 1 encoding the A repeat was deleted. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655). Developed by Takashi Sado, National Institute of Genetics in 2007. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6). Xist<delta A>; B6.Cg-Xist<tm5Sado> Xist<delta A>; B6.Cg-Xist<tm5Sado> true Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.bioreg.kyushu-u.ac.jp/labo/epigenome/index.html" target="_blank">Lab HP (Japanese)</A> Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] Takashi SADO Xist<delta>マウス。Xist遺伝子第1エクソン内のAリピートと呼ばれる領域を欠失する。mutantアレルを♂親から受け継いだ場合、全ての仔のメス (ヘテロ型) は致死となる。しかし、ヘテロのメスから受け継いだ場合は次世代へ伝播することができる。 Phage P1 loxP site, mouse Xist genomic DNA B（1〜3か月） <a href='https://brc.riken.jp/mus/pcr02655'>Genotyping protocol -PCR-</a> 佐渡　敬