B (1-3 months) B（1〜3か月） Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N. RBRC02708 true 理化学研究所脳科学総合研究センター・沢野-阪上朝子先生、宮脇敦史先生。B6D2F1受精卵へTgのインジェクションにより作出。C57BL/6Nへ戻し交配された。 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development (2013) doi:10.1242/dev.099226. The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Coral mKO2 (monomeric Kusabira Orange) cDNA, human Cdt1 cDNA Atsushi MIYAWAKI 宮脇　敦史 B6.Cg-Tg(Fucci)610Bsi, B6;B6D2-Tg(Fucci)610Bsi Carrier x Noncarrier [C57BL/6NJcl] Carrier x Noncarrier [C57BL/6NJcl] <a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/02708_GB.pdf">Genetic Background</a> 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Development (2013) doi:10.1242/dev.099226. <br>学術研究に限る。研究成果の公表にあたって謝辞の表明し、発表内容のコピーを寄託者に送る。pCAGGS expression vector の開発者の大阪大学・宮崎純一（Jun-ichi Miyazaki)博士 (FAX: +81-6-6879-3829、e-mail: jimiyaza@nutri.med.osaka-u.ac.jp) に指定の MTA を提出する。 Carrier x Noncarrier [C57BL/6NJcl] CAGプロモーター制御下で、Fucci (Fluorescent Ubiquitination-based Cell Cycle indicator：フーチ) 蛍光タンパク導入遺伝子を発現するトランスジェニックマウス。細胞周期の進行を蛍光によって観察が可能。細胞周期の可視化ツールとして、個体の発生、分化、再生、がん化など、細胞周期と関連する生命現象の解明に有用なマウス。#474 (RBRC02704) 、#492 (RBRC02705) 、#504 (RBRC02706) ライン：S/G2/M期に発現するGeminin遺伝子と緑色蛍光タンパク (monomeric Azami-Green1: mAG1) を融合した遺伝子を発現する。S/G2/M期の核が緑色の蛍光で観察可能。#596 (RBRC02707) 、#610 (RBRC02708) 、#639 (RBRC02709) ライン：G1期に発現するCdt1遺伝子とオレンジ色の蛍光タンパク (monomeric Kusabira-Orange2: mKO2) を融合した遺伝子を全身で発現する。G1期の核がオレンジ色の蛍光で観察可能。全身での発現が最も優れているラインは、#504および#596 (Cell 2008) 。血球系 (特に単球、マクロファージ系) は、#474および#610 (Development 2013) 。リンパ球系は、#492および#639 (PNAS 2010) 。#474と#610のダブルトランスジェニックマウス (RBRC02817) 、#504と#596のダブルトランスジェニックマウス (RBRC02892) あり。#474および#504：ホモマウスの生存性、繁殖性あり。#596：ホモマウスは恐らく致死 (詳細不明) 。#492、#610および#639：ホモマウスの生存性、繁殖性は不明 (おそらく悪い、詳細不明) 。 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://cfds.riken.jp/material/fucci" target="_blank">Lab HP</A> Fluorescent Proteins/lacZ System Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown). B6.Cg-Tg(FucciG1)#610Bsi B6.Cg-Tg(FucciG1)#610Bsi <a href='https://brc.riken.jp/mus/pcr02708'>Genotyping protocol -PCR-</a>