Heterozygote x Wild-type [C57BL/6JJcl]Homozygotes are embryonic lethal. Mixed (129, B6 and Swiss Webster) Gene targeting was achieved in W4 ES cells RBRC02849 Irel alpa knockout mouse +/- strain:129/C57BL/Black Swiss Webster Irel alpa knockout mouse +/- strain:129/C57BL/Black Swiss Webster Developed by Dr. David Ron, New York University School of Medicine. Gene targeting was achieved in W4 ES cells. David Ron David Ron Mixed (129, B6 and Swiss Webster)Gene targeting was achieved in W4 ES cells 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 287, 664-666 (2000). Nature, 415, 92-96 (2002).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>DEPOSITOR hereby excludes any and all warranties, implied or express, including warranties of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE or of non-infringement of patents or other proprietary right. The sample of BIOLOGOCAL RESOURCE is provided "as is". In no event shall DEPOSITOR be liable for direct, indirect, special, incidental or consequential damages in connection with the BIOLOGICAL RESOURCE. RECIPIENT and RIKEN BRC will indemnify and hold DEPOSITOR harmless from any claims or liability resulting from the use of the BIOLOGICAL RESOURCE by you. Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent form the DEPOSITOR for such application. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 287, 664-666 (2000). Nature, 415, 92-96 (2002).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. DEPOSITOR hereby excludes any and all warranties, implied or express, including warranties of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE or of non-infringement of patents or other proprietary right. The sample of BIOLOGICAL RESOURCE is provided "as is". In no event shall DEPOSITOR be liable for direct, indirect, special, incidental or consequential damages in connection with the BIOLOGICAL RESOURCE. RECIPIENT and RIKEN BRC will indemnify and hold DEPOSITOR harmless from any claims or liability resulting from the use of the BIOLOGICAL RESOURCE by you. Prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent form the DEPOSITOR for such application. B6.Cg-Ern1tm1Dron C（3〜6か月） Heterozygote x Wild-type [C57BL/6JJcl] Homozygotes are embryonic lethal. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> mouse Ern1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin phosphotransferase B6.Cg-Ern1<tm1Dron> true <a href='https://brc.riken.jp/mus/pcr02849'>Genotyping protocol -PCR-</a> Homozygous Ire1alpha- null mice are embryonic lethal C (3-6 months) Ern1, endoplasmic reticulum (ER) to nucleus signaling 1 gene knock-out mice. Homozygous Ern1 mutant mice are embryonic lethal during organogenesis. Diet for breeding is preferable but not essential.