Heterozygote x Wild-type [C57BL/6JJcl] Heterozygote x Wild-type [C57BL/6JJcl] Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> 三菱化学生命科学研究所 池上浩司先生, 瀬藤光利先生。 Mitsutoshi SETOU Ttll1 (TL1) 遺伝子のノックアウトマウス。Ttll1遺伝子のエクソン2と3、および4の一部をネオマイシン耐性遺伝子に置換。開始コドンはエクソン4に存在。相同組み換えにより開始コドンが除去される。ホモで遺伝子の発現なし。 E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter), HSV PolyA signal, mouse TL1 genomic DNA C (3-6 months) C（3〜6か月） 瀬藤　光利 Developed by Ikegami and Mitsutoshi Setou, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were used. The mice were crossed to C57BL/6J. Ttll1 (TL1) gene knockout mice. Exons 2, 3 and a part of exon 4 were replaced with neo cassette. Initiation codon is in exon 4. null mutant. true TL1 KO TL1 KO 条件を付加する。<br>1. 本件リソースの利用は非営利機関による学術研究に限る。<br>2. 本件リソースを非営利機関による学術研究以外の目的で使用する場合には、事前に理研BRCに連絡し、三菱化学（株）と協議する。<br>3. 本件リソースを用いた研究結果を論文投稿、学会発表等により公表する場合には、その由来を明記し、三菱化学（株）に謝意を表明する。<br>4. 理研ＢＲＣは提供先機関名、研究責任者名、利用課題名および提供日を三菱化学（株）に年1回報告する。 <a href='https://brc.riken.jp/mus/pcr03327'>Genotyping protocol -PCR-</a> B6.129-Ttll1<tm1Seto>/SetoRbrc The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.<br>The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.<br>In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.<br>The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution. TL1 KO RBRC03327