Developed by Shinobu Fujita, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were used. The mice were crossed to C57BL/6. human APOE3 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA Cre/loxP system true ApoE3-7 KO D（6か月以上） Hematological Research The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.<br>The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.<br>In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.<br>The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution. D (more than 6 months) Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Human ApoE3 knock-in mice line 7. The mouse ApoE sequences including part of exon 2, entire exon 3 and most of exon 4 was replaced by the human APOE3 and a floxed neo casette. Cardiovascular Research RBRC03391 Heterozygote x Wild-type [C57BL/6NJcl] Heterozygote x Wild-type [C57BL/6NJcl] ApoE3-7 KO ApoE3-7 KO 藤田　忍 条件を付加する。<br>1. 本件リソースの利用は非営利機関による学術研究に限る。<br>2. 本件リソースを非営利機関による学術研究以外の目的で使用する場合には、事前に理研BRCに連絡し、三菱化学（株）と協議する。<br>3. 本件リソースを用いた研究結果を論文投稿、学会発表等により公表する場合には、その由来を明記し、三菱化学（株）に謝意を表明する。<br>4. 理研ＢＲＣは提供先機関名、研究責任者名、利用課題名および提供日を三菱化学（株）に年1回報告する。 作出系統：129, C57BL/6N表現型：マウスアポEの替わりにヒトアポE3を発現三菱化学生命科学研究所 藤田 忍先生。 Shinobu FUJITA マウスApoe遺伝子のエクソン2の19bp以降をヒトApoE3 cDNAおよびfloxed neo耐性遺伝子で置換したノックインマウス。マウスApoEの代わりにヒトApoE3を発現する。ライン7。 作出系統：129, C57BL/6N 表現型：マウスアポEの替わりにヒトアポE3を発現 三菱化学生命科学研究所 藤田 忍先生。 B6;129-Apoe<tm>/7SfuRbrc