RBRC03454 <a href='https://brc.riken.jp/mus/pcr03454'>Genotyping protocol -PCR-</a> Mvh KO (=Vas2) The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.<br>The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.<br>In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.<br>The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution. Mvh KO (=Vas2) 作出系統：129: C57BL6J遺伝背景： C57BL6J/129兄妹交配F3-4表現型：♂ホモで不妊毛色：black/Agouti 条件を付加する。<br>1. 本件リソースの利用は非営利機関による学術研究に限る。<br>2. 本件リソースを非営利機関による学術研究以外の目的で使用する場合には、事前に理研BRCに連絡し、三菱化学（株）と協議する。<br>3. 本件リソースを用いた研究結果を論文投稿、学会発表等により公表する場合には、その由来を明記し、三菱化学（株）に謝意を表明する。<br>4. 理研ＢＲＣは提供先機関名、研究責任者名、利用課題名および提供日を三菱化学（株）に年1回報告する。 Fluorescent Proteins/lacZ System true Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Toshiaki NOSE Mvh KO (=Vas2) Mvh KO (=Vas2) 作出系統：129: C57BL6J 遺伝背景： C57BL6J/129兄妹交配F3-4 表現型：♂ホモで不妊 毛色：black/Agouti 野瀬　俊明 C (3-6 months) C（3〜6か月） Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Ddx4 genomic DNA