Yasuyoshi ARIMATSU Lxn KO Lxn KO The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.<br>The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.<br>In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.<br>The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution. true RBRC03456 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Developed by Yasuyoshi Arimatsu, Mitsubishi Kagaku Institute of Life Sciences. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6N. Lxn (latexin) gene knockout mice. Exon 1 of the Lxn gene was replaced with a neomycin resistant cassette. 三菱化学生命科学研究所・有松靖温先生。D3 ES細胞を用いて作出。C57BL/6へ戻し交配された。 Lxn KO C (3-6 months) <a href='https://brc.riken.jp/mus/pcr03456'>Genotyping protocol -PCR-</a> Lxn (latexin) 遺伝子のノックアウトマウス。エクソン1をneoカセットで置換。 C(3〜6か月) mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Lxn genomic DNA 条件を付加する。<br>1. 本件リソースの利用は非営利機関による学術研究に限る。<br>2. 本件リソースを非営利機関による学術研究以外の目的で使用する場合には、事前に理研BRCに連絡し、三菱化学(株)と協議する。<br>3. 本件リソースを用いた研究結果を論文投稿、学会発表等により公表する場合には、その由来を明記し、三菱化学(株)に謝意を表明する。<br>4. 理研BRCは提供先機関名、研究責任者名、利用課題名および提供日を三菱化学(株)に年1回報告する。 有松 靖温 Heterozygote x Wild-type [C57BL/6NJcl] Heterozygote x Wild-type [C57BL/6NJcl] B6N.129S2-Lxn<tm1Yari>/YariRbrc