An EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are neonatal lethal due to cleft palate. A loxP flanked neo was removed by crossing with cre mice. 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J. Comp Neurol., 467, 60-79 (2003).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>本件リソースを寄託者の許可なしに他者に譲渡しない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 Gad1 (GAD67) 遺伝子のGFPノックインマウス。Gad1遺伝子のエクソン1のATGコドンにEGFPが挿入されている。ホモマウスは出生後致死 (口蓋裂により死亡) 。loxPで挟まれたNeomycin耐性遺伝子はcreマウスとの交配により除去されている。 Jellyfish green fluorescent protein cDNA, phage P1loxP site, mouse Gad1 genomic DNA C (3-6 months) RBRC03674 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp Neurol., 467, 60-79 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without DEPOSITOT's permission. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. C（3〜6か月） 群馬大学大学院医学研究科・柳川右千夫先生。TT2 ES細胞由来。ICR混合背景。 Cre/loxP system <a href='https://brc.riken.jp/mus/pcr03674'>Genotyping protocol -PCR-</a> Developed by Yuchio Yanagawa, Graduate School of Medicine, Gumma University. ICR mixed background. TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] 柳川　右千夫 Fluorescent Proteins/lacZ System GAD67-GFP dNeo KI GAD67-GFP dNeo KI ICR.Cg-Gad1<tm1.1Tama> ICR.Cg-Gad1<tm1.1Tama> <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jan_2014_mm" target="_blank">Mouse of the Month Jan 2014</A> Yuchio YANAGAWA Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> Gad1 (GAD67) 遺伝子のGFPノックインマウス。Gad1遺伝子のエクソン1のATGコドンにEGFPが挿入されている。ホモマウスは出生後致死 (口蓋裂により死亡) 。loxPで挟まれたNeomycin耐性遺伝子はcreマウスとの交配により除去されている。, Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)] Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)] true