Carrier x Noncarrier [or Crossing to Jcl:B6D2F1] B6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb B6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb 開発者/機関 : 岡部勝先生/大阪大学 開発年 : 2003年 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 59:105-107(2010).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. RBRC03743 B6D2F1-Tg(CAG/su9-DsRed2,Acr3-EGFP), RBGS002Osb (Nov. 2011), RBGS-002 B6D2F1-Tg(CAG/su9-DsRed2,Acr3-EGFP), RBGS002Osb (Nov. 2011), RBGS-002 B (1-3 months) Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/tbusa_mta.docx">FP license of TBUSA</A></li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol><A HREF="https://egr.biken.osaka-u.ac.jp/achievement/bio_resources" target="_blank">Lab HP</A> Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with Acr3-EGFP and CAG/su9-DsRed2 transgene constructs into fertilized eggs. C57BL/6 and DBA/2 mixed background. 開発者/機関 : 岡部勝先生/大阪大学開発年 : 2003年 true BDF1と交配して維持 <a href='https://brc.riken.jp/mus/pcr03743'>Genotyping protocol -PCR-</a> CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)], Discosoma sp. DsRed2 cDNA, acrosin promoter, acrosin localization signal, jellyfish EGFP cDNA Fluorescent Proteins/lacZ System 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Exp. Anim., 59:105-107(2010).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること（https://www.ccb.osaka-u.ac.jp/en/）。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び事業活動を行う場合は、寄託者と別途協議を行う。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 精子のアクロソームにGFP、ミトコンドリアにDsRed2の蛍光をもつ。,アクロソーム反応前の精子は緑と赤、反応後の精子は赤の蛍光をもつ。,in vitro, in vivoの受精時におけるイメージング等に有用である。,遺伝子の検出方法 : ,蛍光観察 (DsRed2の蛍光を実体蛍光顕微鏡・倒立蛍光顕微鏡下で観察),560nmの励起で全身が赤く、480nmで励起すれば精子の先体が緑に光る。 B（1〜3か月） Masaru OKABE Trangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9). 精子のアクロソームにGFP、ミトコンドリアにDsRed2の蛍光をもつ。アクロソーム反応前の精子は緑と赤、反応後の精子は赤の蛍光をもつ。in vitro, in vivoの受精時におけるイメージング等に有用である。遺伝子の検出方法 : 蛍光観察 (DsRed2の蛍光を実体蛍光顕微鏡・倒立蛍光顕微鏡下で観察)560nmの励起で全身が赤く、480nmで励起すれば精子の先体が緑に光る。 岡部　勝